J Toxicol Sci. 2010 Apr;35(2):253-6. doi: 10.2131/jts.35.253.
Quantitative analysis of metabolites is important in (1)H-nuclear magnetic resonance (NMR)-based metabolomics of plasma. Human plasma contains a high density of proteins which heavily adsorb the commonly-used standard compound of sodium 3-(trimethylsilyl) propionate 2, 2, 3, 3-d(4) (TSP). We have evaluated calcium formate as an alternative standard in 1D single-pulse (1)H-NMR spectra to quantify plasma metabolites. Formate did not interact with either plasma metabolites or proteins under adequate conditions. Linear relations between the signal intensities and the added formate have been demonstrated in (1)H spectra. The quantifications of glucose and creatinine by this method have shown good accordance with biochemical analysis. Calcium formate is applicable as a concentration standard to NMR metabolomics of plasma.
在基于（1）H 核磁共振（NMR）的血浆代谢组学中，对代谢物进行定量分析非常重要。人类血浆中含有高密度的蛋白质，这些蛋白质会强烈吸附常用的标准化合物 3-(三甲基硅基)丙酸钠 2, 2, 3, 3-d(4) (TSP)。我们评估了甲酸钙作为替代标准，用于 1D 单脉冲（1）H-NMR 光谱定量测定血浆代谢物。在适当的条件下，甲酸不会与血浆代谢物或蛋白质相互作用。在（1）H 光谱中已经证明了信号强度与添加的甲酸之间存在线性关系。该方法对葡萄糖和肌酐的定量与生化分析结果具有良好的一致性。甲酸钙可作为浓度标准应用于血浆 NMR 代谢组学。
