The Key Laboratory of Forest Genetics and Biotechnology and Jiangsu Key Laboratory for Poplar Germplasm Enhancement and Variety Improvement, Nanjing Forestry University, Nanjing 210037, China.
J Microbiol Biotechnol. 2010 Mar;20(3):474-9.
In this paper, we analyzed the microsatellites in the transcript sequences of the whole Laccaria bicolor genome. Our results revealed that, apart from the triplet repeats, length diversification and richness of the detected microsatellites positively correlated with their repeat motif lengths, which were distinct from the variation trends observed for the transcriptional microsatellites in the genome of high plant. We also compare the microsatellites detected in the genic regions and in the non-genic regions of the L. bicolor genome. Subsequently, SSR primers were designed for the transcriptional microsatellites in the L. bicolor genome. These SSR primers provide desirable genetic resources to the ectomycorrhizae community and this study provides deep insight into the characteristics of the microsatellite sequences in the L. bicolor genome.
在本文中，我们分析了整个双色蜡蘑基因组转录序列中的微卫星。我们的结果表明，除了三核苷酸重复外，检测到的微卫星的长度多样性和丰富度与它们的重复基序长度呈正相关，这与在高等植物基因组中观察到的转录微卫星的变化趋势不同。我们还比较了双色蜡蘑基因组中基因区域和非基因区域中检测到的微卫星。随后，为双色蜡蘑基因组中的转录微卫星设计了 SSR 引物。这些 SSR 引物为外生菌根社区提供了理想的遗传资源，本研究深入了解了双色蜡蘑基因组中微卫星序列的特征。
