利用光谱分解进行脂质的高速非线性干涉振动分析。
High speed nonlinear interferometric vibrational analysis of lipids by spectral decomposition.
机构信息
Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, USA.
出版信息
Anal Chem. 2010 May 1;82(9):3812-8. doi: 10.1021/ac100222c.
Abstract
Unlike other CARS-based (coherent anti-Stokes Raman scattering) spectroscopy techniques, nonlinear interferometric vibrational spectroscopy (NIVS) is linear in analyte concentration and has a Raman line shape free of nonresonant background distortions. We use spontaneous Raman scattering as a high accuracy benchmark for NIVS. As a challenging comparison, we examine spectra in the CH stretching region of six lipid samples. Singular value decomposition and reference to an independent chemical assay are used to directly compare NIVS and spontaneous Raman scattering. We demonstrate that NIVS can determine the relative degree of unsaturation in six different lipid samples as accurately as spontaneous Raman spectroscopy, but 200 times faster. A skin tissue sample is mapped out to demonstrate quantitative lipid-protein differentiation with spatial resolution.
摘要
与其他基于 CARS 的（相干反斯托克斯拉曼散射）光谱技术不同，非线性干涉振动光谱（NIVS）在分析物浓度上是线性的，并且拉曼谱线形状没有非共振背景扭曲。我们使用自发拉曼散射作为 NIVS 的高精度基准。作为一个具有挑战性的比较，我们检查了六种脂质样品的 CH 伸缩区域的光谱。奇异值分解和对独立化学分析的参考用于直接比较 NIVS 和自发拉曼散射。我们证明，NIVS 可以像自发拉曼光谱一样准确地确定六种不同脂质样品的不饱和程度，但速度快 200 倍。对皮肤组织样本进行映射，以证明具有空间分辨率的定量脂质-蛋白质分化。
相似文献
1
High speed nonlinear interferometric vibrational analysis of lipids by spectral decomposition.利用光谱分解进行脂质的高速非线性干涉振动分析。
Anal Chem. 2010 May 1;82(9):3812-8. doi: 10.1021/ac100222c.
2
Fast vibrational imaging of single cells and tissues by stimulated Raman scattering microscopy.通过受激拉曼散射显微镜对单细胞和组织进行快速振动成像。
Acc Chem Res. 2014 Aug 19;47(8):2282-90. doi: 10.1021/ar400331q. Epub 2014 May 28.
3
Spectral interferometric implementation with passive polarization optics of coherent anti-Stokes Raman scattering.利用相干反斯托克斯拉曼散射的被动偏振光学进行光谱干涉测量。
Phys Rev Lett. 2013 Sep 6;111(10):103902. doi: 10.1103/PhysRevLett.111.103902. Epub 2013 Sep 5.
4
Nonlinear interferometric vibrational imaging.非线性干涉振动成像。
Phys Rev Lett. 2004 Mar 26;92(12):123905. doi: 10.1103/PhysRevLett.92.123905. Epub 2004 Mar 25.
5
Nonlinear interferometric vibrational imaging for fast label-free visualization of molecular domains in skin.非线性干涉振动成像技术可快速实现皮肤中分子域的无标记可视化。
Anal Bioanal Chem. 2011 Jul;400(9):2817-25. doi: 10.1007/s00216-011-4953-z. Epub 2011 Apr 5.
6
Fourier transform spectral interferometric coherent anti-Stokes Raman scattering (FTSI-CARS) spectroscopy.傅里叶变换光谱干涉相干反斯托克斯拉曼散射（FTSI-CARS）光谱学
Opt Lett. 2007 May 15;32(10):1332-4. doi: 10.1364/ol.32.001332.
7
High-Speed Nonlinear Interferometric Vibrational Imaging of Biological Tissue With Comparison to Raman Microscopy.生物组织的高速非线性干涉振动成像及其与拉曼显微镜的比较
IEEE J Quantum Electron. 2009 Dec 4;16(4):824-832. doi: 10.1109/JSTQE.2009.2035537.
8
Single-pulse coherent anti-Stokes Raman scattering microscopy employing an octave spanning pulse.采用倍频程跨度脉冲的单脉冲相干反斯托克斯拉曼散射显微镜。
Opt Express. 2009 Jul 6;17(14):11259-66. doi: 10.1364/oe.17.011259.
9
Quantitative chemical imaging with background-free multiplex coherent anti-Stokes Raman scattering by dual-soliton Stokes pulses.利用双孤子斯托克斯脉冲进行无背景多重相干反斯托克斯拉曼散射的定量化学成像。
Biomed Opt Express. 2016 Sep 9;7(10):3927-3939. doi: 10.1364/BOE.7.003927. eCollection 2016 Oct 1.
10
High-spectral-resolution coherent anti-Stokes Raman scattering with interferometrically detected broadband chirped pulses.采用干涉探测宽带啁啾脉冲的高光谱分辨率相干反斯托克斯拉曼散射。
Opt Lett. 2006 May 15;31(10):1543-5. doi: 10.1364/ol.31.001543.
引用本文的文献
1
Tracking the formation and degradation of fatty-acid-accumulated mitochondria using label-free chemical imaging.使用无标记化学成像技术追踪脂肪酸积累线粒体的形成和降解。
Sci Rep. 2021 Mar 23;11(1):6671. doi: 10.1038/s41598-021-85795-1.
2
Stimulated Raman scattering spectroscopic optical coherence tomography.受激拉曼散射光谱光学相干断层扫描术
Optica. 2017 Feb;4(2):243-246. doi: 10.1364/OPTICA.4.000243. Epub 2017 Feb 14.
3
Dispersion-based stimulated Raman scattering spectroscopy, holography, and optical coherence tomography.基于色散的受激拉曼散射光谱学、全息术和光学相干断层扫描术。
Opt Express. 2016 Jan 11;24(1):485-98. doi: 10.1364/OE.24.000485.
4
Biological imaging with coherent Raman scattering microscopy: a tutorial.相干拉曼散射显微镜的生物成像：教程
J Biomed Opt. 2014 Jul;19(7):71407. doi: 10.1117/1.JBO.19.7.071407.
5
The Gold Nanorod-Biology Interface: From Proteins to Cells to Tissue.金纳米棒与生物的界面：从蛋白质到细胞再到组织
Curr Phys Chem. 2013 Apr 1;3(2). doi: 10.2174/1877946811303020002.
6
Label-free quantitative imaging of cholesterol in intact tissues by hyperspectral stimulated Raman scattering microscopy.利用高光谱受激拉曼散射显微镜对完整组织中的胆固醇进行无标记定量成像。
Angew Chem Int Ed Engl. 2013 Dec 2;52(49):13042-6. doi: 10.1002/anie.201306234. Epub 2013 Oct 14.
7
Biomolecular imaging with coherent nonlinear vibrational microscopy.基于相干非线性振动显微镜的生物分子成像
Annu Rev Phys Chem. 2013;64:77-99. doi: 10.1146/annurev-physchem-040412-110103. Epub 2012 Dec 5.
8
Quantitative vibrational imaging by hyperspectral stimulated Raman scattering microscopy and multivariate curve resolution analysis.基于高光谱受激拉曼散射显微镜和多变量曲线分辨分析的定量振动成像。
Anal Chem. 2013 Jan 2;85(1):98-106. doi: 10.1021/ac3019119. Epub 2012 Dec 14.
9
Characterization of cholesterol crystals in atherosclerotic plaques using stimulated Raman scattering and second-harmonic generation microscopy.应用受激拉曼散射和二次谐波产生显微镜对动脉粥样硬化斑块中的胆固醇晶体进行表征。
Biophys J. 2012 Apr 18;102(8):1988-95. doi: 10.1016/j.bpj.2012.03.016.
10
Identification of cholesterol crystals in plaques of atherosclerotic mice using hyperspectral CARS imaging.利用高光谱 CARS 成像技术鉴定动脉粥样硬化小鼠斑块中的胆固醇晶体。
J Lipid Res. 2011 Dec;52(12):2177-2186. doi: 10.1194/jlr.M018077. Epub 2011 Sep 23.
本文引用的文献
1
Coherent anti-stokes Raman scattering microscopy: chemical imaging for biology and medicine.相干反斯托克斯拉曼散射显微镜：生物学和医学中的化学成像。
Annu Rev Anal Chem (Palo Alto Calif). 2008;1:883-909. doi: 10.1146/annurev.anchem.1.031207.112754.
2
Chasing lipids in health and diseases by coherent anti-Stokes Raman scattering microscopy.利用相干反斯托克斯拉曼散射显微镜在健康与疾病中追踪脂质
Vib Spectrosc. 2009 May 26;50(1):160-167. doi: 10.1016/j.vibspec.2008.11.007.
3
One-laser interferometric broadband coherent anti-Stokes Raman scattering.单激光干涉宽带相干反斯托克斯拉曼散射
Opt Express. 2006 Apr 17;14(8):3631-40. doi: 10.1364/oe.14.003631.
4
Direct extraction of Raman line-shapes from congested CARS spectra.从拥挤的相干反斯托克斯拉曼散射（CARS）光谱中直接提取拉曼线形。
Opt Express. 2006 Apr 17;14(8):3622-30. doi: 10.1364/oe.14.003622.
5
Raman and CARS microspectroscopy of cells and tissues.细胞与组织的拉曼和 CARS 微光谱学。
Analyst. 2009 Jun;134(6):1046-57. doi: 10.1039/b822354h. Epub 2009 Feb 26.
6
Chemical imaging of oral solid dosage forms and changes upon dissolution using coherent anti-Stokes Raman scattering microscopy.利用相干反斯托克斯拉曼散射显微镜对口服固体制剂及其溶解过程中的化学成像。
Anal Chem. 2009 Mar 15;81(6):2085-91. doi: 10.1021/ac8020856.
7
Coherent anti-Stokes Raman scattering imaging of lipids in cancer metastasis.癌症转移中脂质的相干反斯托克斯拉曼散射成像
BMC Cancer. 2009 Jan 30;9:42. doi: 10.1186/1471-2407-9-42.
8
Single-shot interferometric approach to background free broadband coherent anti-Stokes Raman scattering spectroscopy.用于无背景宽带相干反斯托克斯拉曼散射光谱的单次干涉测量方法。
Opt Express. 2009 Jan 5;17(1):123-35. doi: 10.1364/oe.17.000123.
9
Label-free biomedical imaging with high sensitivity by stimulated Raman scattering microscopy.基于受激拉曼散射显微镜的高灵敏度无标记生物医学成像。
Science. 2008 Dec 19;322(5909):1857-61. doi: 10.1126/science.1165758.
10
Polarization coherent anti-Stokes Raman scattering microscopy.偏振相干反斯托克斯拉曼散射显微镜术
Opt Lett. 2001 Sep 1;26(17):1341-3. doi: 10.1364/ol.26.001341.
Zotero 插件