利用相干反斯托克斯拉曼散射显微镜在健康与疾病中追踪脂质
Chasing lipids in health and diseases by coherent anti-Stokes Raman scattering microscopy.
作者信息
Wang Han-Wei, Fu Yan, Huff Terry B, Le Thuc T, Wang Haifeng, Cheng Ji-Xin
机构信息
Weldon School of Biomedical Engineering, Purdue University, West Lafayette, IN 47907, USA.
出版信息
Vib Spectrosc. 2009 May 26;50(1):160-167. doi: 10.1016/j.vibspec.2008.11.007.
Abstract
The integration of near IR picosecond pulse excitation, collinear beam geometry, epi-detection, and laser-scanning has produced a coherent anti-Stokes Raman scattering (CARS) microscope with a detection sensitivity of 10(5) vibrational oscillators, sub-micron 3D resolution, and video-rate acquisition speed. The incorporation of spectral detection and other imaging modalities has added versatility to the CARS microscope. These advances allowed sensitive interrogation of biological samples, particularly lipids that have a high density of CH(2) groups. With initial applications to membrane domains, lipid bodies, demyelinating diseases, obesity, and cardiovascular diseases, CARS microscopy is poised to become a powerful bio-imaging tool with the availability of a multifunctional, affordable, easy-to-operate CARS microscope, and the development of CARS endoscopy for in vivo diagnosis.
摘要
近红外皮秒脉冲激发、共线光束几何结构、落射检测和激光扫描的结合，产生了一种相干反斯托克斯拉曼散射（CARS）显微镜，其检测灵敏度为10^5个振动振子，具有亚微米级三维分辨率和视频速率采集速度。光谱检测和其他成像方式的加入增加了CARS显微镜的多功能性。这些进展使得能够对生物样品进行灵敏检测，特别是对具有高密度CH₂基团的脂质。随着CARS显微镜最初应用于膜结构域、脂质体、脱髓鞘疾病、肥胖症和心血管疾病，随着多功能、价格合理且易于操作的CARS显微镜的问世以及用于体内诊断的CARS内窥镜的开发，CARS显微镜有望成为一种强大的生物成像工具。
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