Department of Cell and Developmental Biology, University of Pennsylvania, Philadelphia, PA 19104, USA.
Nucleic Acids Res. 2010 Aug;38(14):4599-606. doi: 10.1093/nar/gkq218. Epub 2010 Apr 7.
We previously showed that histone H4 serine-1 phosphorylation (H4S1ph) is evolutionarily conserved during gametogenesis, and contributes to post-meiotic nuclear compaction and to full completion of sporulation in the yeast Saccharomyces cerevisiae. Previous studies showed that H4S1ph and another modification of the same histone, H4 acetylation (H4ac), do not occur together and have opposing roles during DNA double-strand break (DSB) repair. In this study, we investigated the relationship between these marks during yeast sporulation. H4S1ph and H4ac co-exist globally during later stages of sporulation, in contrast to DSB repair. Genome-wide mapping during sporulation reveals accumulation of both marks over promoters of genes. Prevention of H4S1ph deposition delays the decline in transcription that normally occurs during spore maturation. Taken together, our results indicate that H4S1ph deposition reinforces reduced transcription that coincides with full spore compaction, without disrupting the local acetylation signature. These studies indicate distinctive features of a histone H4 modification marking system during sporulation compared with DSB repair.
我们之前已经证明，组蛋白 H4 丝氨酸 1 磷酸化（H4S1ph）在配子发生过程中是进化保守的，并且有助于减数分裂后核的紧密化以及酵母酿酒酵母中孢子形成的完全完成。先前的研究表明，H4S1ph 和同一组蛋白的另一种修饰，H4 乙酰化（H4ac），在 DNA 双链断裂（DSB）修复过程中不会同时发生，并且具有相反的作用。在这项研究中，我们研究了酵母孢子形成过程中这些标记之间的关系。H4S1ph 和 H4ac 在孢子形成的后期阶段全局共存，与 DSB 修复相反。在孢子形成过程中的全基因组图谱揭示了这两种标记在基因启动子上的积累。防止 H4S1ph 沉积会延迟通常在孢子成熟过程中发生的转录下降。总的来说，我们的结果表明，H4S1ph 沉积加强了与完全孢子压缩同时发生的转录减少，而不会破坏局部乙酰化特征。这些研究表明，与 DSB 修复相比，组蛋白 H4 修饰标记系统在孢子形成过程中有独特的特征。
