分光光度法同时测定片剂剂型中草酸艾司西酞普兰和氯硝西泮的含量。

Spectrophotometric method for simultaneous estimation of escitalopram oxalate and clonazepam in tablet dosage form.

作者信息

Kakde R B, Satone D D

机构信息

Department of Pharmaceutical Sciences, Rashtrasant Tukadoji Maharaj Nagpur University, Nagpur-440 033, India.

出版信息

Indian J Pharm Sci. 2009 Nov;71(6):702-5. doi: 10.4103/0250-474X.59559.

DOI:10.4103/0250-474X.59559

PMID:20376230

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2846482/

Abstract

A simple, accurate and precise spectrophotometric method has been developed for simultaneous estimation of escitalopram oxalate and clonazepam in combined dosage form. Simultaneous equation method is employed for simultaneous determination of escitalopram oxalate and clonazepam from combined dosage forms. In this method, the absorbance was measured at 238 nm for escitalopram oxalate and 273 nm for clonazepam. Linearity was observed in range of 5-100 mug/ml and 5-50 mug/ml for escitalopram and clonazepam respectively. Recovery studies confirmed the accuracy of proposed method and results were validated as per ICH guidelines. The method can be used for routine quality control of pharmaceutical formulation containing escitalopram and clonazepam.

摘要

已开发出一种简单、准确且精密的分光光度法，用于同时测定复方制剂中草酸艾司西酞普兰和氯硝西泮的含量。采用联立方程法从复方制剂中同时测定草酸艾司西酞普兰和氯硝西泮。在此方法中，草酸艾司西酞普兰在238 nm处测定吸光度，氯硝西泮在273 nm处测定吸光度。草酸艾司西酞普兰和氯硝西泮的线性范围分别为5 - 100 μg/ml和5 - 50 μg/ml。回收率研究证实了该方法的准确性，结果按照ICH指南进行了验证。该方法可用于含草酸艾司西酞普兰和氯硝西泮的药物制剂的常规质量控制。