抗生素化学分析的改进。十七、氨基柱在肉类、鱼类和蛋类中残留磺胺类抗菌药物测定中的应用。

Improvement of chemical analysis of antibiotics. XVII. Application of an amino cartridge to the determination of residual sulphonamide antibacterials in meat, fish and egg.

作者信息

Ikai Y, Oka H, Kawamura N, Hayakawa J, Yamada M, Harada K, Suzuki M, Nakazawa H

机构信息

Aichi Prefectural Institute of Public Health, Nagoya, Japan.

出版信息

J Chromatogr. 1991 Mar 22;541(1-2):393-400. doi: 10.1016/s0021-9673(01)96011-x.

DOI:10.1016/s0021-9673(01)96011-x

PMID:2037655

Abstract

A simple, rapid and reliable method for the determination of residual sulphonamide antibacterials (SAs) (sulphathiazole, sulphisozole, sulphamethoxazole, sulphadiazine, sulphamerazine, sulphadimidine, sulphamonomethoxine, sulphadimethoxine, sulphamethoxypyridazine and sulphaquinoxaline) in meat, fish and egg was developed using a combination of high-performance liquid chromatography (HPLC) and clean-up with an amino-type prepacked cartridge. SAs were extracted with ethyl acetate and applied to a Baker 10 amino cartridge. After elution from the cartridge, SAs were determined by HPLC. The recoveries at the level of 0.5 ppm were 73.7-99.1% and the detection limits were 0.05 ppm. The analysis time per sample was about 45 min.

摘要

建立了一种简单、快速且可靠的方法，用于测定肉、鱼和蛋中的残留磺胺类抗菌药物（SAs）（磺胺噻唑、磺胺异噁唑、磺胺甲恶唑、磺胺嘧啶、磺胺甲基嘧啶、磺胺二甲嘧啶、磺胺间甲氧嘧啶、磺胺二甲氧嘧啶、磺胺甲氧嗪和磺胺喹恶啉）。该方法结合了高效液相色谱法（HPLC）和使用氨基型预填充柱进行净化。SAs用乙酸乙酯提取后应用于贝克10氨基柱。从柱上洗脱后，通过HPLC测定SAs。0.5 ppm水平的回收率为73.7 - 99.1%，检测限为0.05 ppm。每个样品的分析时间约为45分钟。