建立实时和嵌套 RT-PCR 检测星状病毒的方法及其在中国江门市的一年流行情况调查。

Development of real-time and nested RT-PCR to detect astrovirus and one-year survey of astrovirus in Jiangmen City, China.

机构信息

Department of Epidemiology, School of Public Health and Tropical Medicine, Southern Medical University, Tonghe Road, Guangzhou, Guang Dong, People's Republic of China.

出版信息

Arch Virol. 2010 Jun;155(6):977-82. doi: 10.1007/s00705-010-0664-6. Epub 2010 Apr 9.

DOI:10.1007/s00705-010-0664-6

PMID:20376681

Abstract

Human astroviruses (HAstVs) are one of the major viral agents of acute gastroenteritis (AGE) in all age groups, especially in young children. In this study, new one-step real-time RT-PCR and nested RT-PCR assays were developed to detect HAstVs. HAstVs were identified in 46 (8.75%) of 526 stool samples in Jiangmen City over 1 year, including 43 (9.15%) of 470 children and 3 (5.4%) of 56 adults, and HAstV-1 was the most predominant strain. This finding suggests that HAstVs infections are common in Jiangmen City, China. Further detailed molecular epidemiological studies are required for understanding the prevalence of HAstVs infection and gaining knowledge about the circulating genotypes.

摘要

人星状病毒（HAstVs）是各年龄段急性胃肠炎（AGE）的主要病毒病原体之一，尤其在幼儿中更为常见。在本研究中，开发了新的一步法实时 RT-PCR 和巢式 RT-PCR 检测方法来检测 HAstVs。在江门市 1 年的 526 份粪便样本中，共检出 46 份（8.75%）HAstV，其中 470 名儿童中有 43 份（9.15%），56 名成人中有 3 份（5.4%），且以 HAstV-1 型最为常见。这表明在中国江门市，HAstV 感染较为常见。需要进一步开展详细的分子流行病学研究，以了解 HAstV 感染的流行情况，并获得有关流行基因型的知识。