Thelu J, Sheick-Zakiuddin I, Boudin C, Peyron F, Picot S, Ambroise-Thomas P
Département de Parasitologie, Centre National de la Recherche Scientifique URA 1344, Faculté de Médecine, Université Joseph Fourier Grenoble 1, La Tronche, France.
J Clin Microbiol. 1991 Mar;29(3):510-8. doi: 10.1128/jcm.29.3.510-518.1991.
A longitudinal study was carried out in Burkina Faso to investigate the natural development of the immune response to Plasmodium falciparum malaria. Three bleedings were carried out before, during, and after the seasonal peak of transmission. Detailed antigen mapping and antibody prevalence of the 248 collected serum samples were established by immunoblotting on the basis of several epidemiological and biological parameters. An improved Western immunoblotting system was used to analyze up to 67 serum samples on each nitrocellulose sheet. This system allowed us to perform the entire study with strictly comparable conditions. Two different blood-stage antigens (exoantigens and somatic antigens) were used to analyze the distribution of different classes and subclasses of immunoglobulins according to the age of the individuals, the presence or absence of a malarial attack, the transmission period, the origin of parasite isolates, and the response to intraerythrocytic stages. Although this analysis emphasizes strong individual variations, reactions with two major antigens of 115 and 103 kDa were especially noted. These antigens induced high antibody levels and prevalences but were probably not involved in protection. The prevalence of immunoglobulin G (IgG) antibodies differed by isotype. Most of antigens stimulating IgG production were also responsible for the IgM antibody response. The role played by these antibodies in the development of natural immunity against malaria is discussed.
在布基纳法索开展了一项纵向研究，以调查对恶性疟原虫疟疾免疫反应的自然发展情况。在传播季节高峰之前、期间和之后进行了三次采血。根据若干流行病学和生物学参数，通过免疫印迹法确定了所采集的248份血清样本的详细抗原图谱和抗体流行情况。使用一种改进的Western免疫印迹系统，在每张硝酸纤维素膜上分析多达67份血清样本。该系统使我们能够在严格可比的条件下进行整个研究。使用两种不同的血液阶段抗原（外抗原和体细胞抗原），根据个体年龄、是否有疟疾发作、传播期、寄生虫分离株的来源以及对红细胞内期的反应，分析免疫球蛋白不同类别和亚类的分布。尽管该分析强调了个体差异很大，但特别注意到与两种主要抗原（115 kDa和103 kDa）的反应。这些抗原诱导了高抗体水平和流行率，但可能不参与保护作用。免疫球蛋白G（IgG）抗体的流行率因同种型而异。大多数刺激IgG产生的抗原也引发了IgM抗体反应。讨论了这些抗体在疟疾自然免疫发展中所起的作用。
