Vidal M F, Gilman R H, Ungar B L, Verástegui M R, Benel A C, Marquis G, Penny M, Lanata C, Miranda E
Department of Microbiology, Universidad Peruana Cayetano Heredia, Lima.
J Clin Microbiol. 1991 Mar;29(3):636-7. doi: 10.1128/jcm.29.3.636-637.1991.
Stool microscopy and an enzyme-linked immunosorbent assay (ELISA) for Giardia lamblia antigen detection were compared for detecting G. lamblia in 30 Peruvian infants. Of 1,131 fecal specimens, G. lamblia was detected by ELISA alone in 44, by microscopy alone in 17, and by both methods in 91. In another group of 17 children negative for G. lamblia by stool microscopy, 6 had G. lamblia detected by ELISA or duodenal aspiration: 2 only by ELISA, 1 only by duodenal aspirate examination, and 3 by both examinations. The ELISA is useful for the detection of G. lamblia in fecal specimens but compared to stool microscopy does not significantly increase the detection of cases.
对30名秘鲁婴儿粪便中的蓝氏贾第鞭毛虫进行检测,比较了粪便显微镜检查和用于检测蓝氏贾第鞭毛虫抗原的酶联免疫吸附测定(ELISA)。在1131份粪便标本中,仅通过ELISA检测出蓝氏贾第鞭毛虫的有44份,仅通过显微镜检查检测出的有17份,两种方法均检测出的有91份。在另一组17名粪便显微镜检查未检测出蓝氏贾第鞭毛虫的儿童中,有6名通过ELISA或十二指肠抽吸检测出蓝氏贾第鞭毛虫:仅通过ELISA检测出的有2名,仅通过十二指肠抽吸检查检测出的有1名,两种检查均检测出的有3名。ELISA可用于检测粪便标本中的蓝氏贾第鞭毛虫,但与粪便显微镜检查相比,并未显著增加病例的检出率。