Araki Yuko, Karita Shuichi, Tsuchiya Tohru, Kondo Makoto, Goto Masakazu
Graduate School of Bioresources, Mie University, Tsu, Japan.
Biosci Biotechnol Biochem. 2010;74(4):802-5. doi: 10.1271/bbb.90845. Epub 2010 Apr 7.
Endoglucanase Cel5A from Clostridium josui contains a family 17 carbohydrate-binding module (CBM) (CjCBM17) and a family 28 CBM (CjCBM28) in tandem. These two CBMs bound to non-crystalline cellulose and beta-1,3-1,4-glucan. Our results indicate that the CBMs recognized different components on the cell wall of a sweet potato root. The root was cut into longitudinal sections. We used CjCBM17 and CjCBM28 fused to two different fluorescent proteins to visualize differential recognition of the plant cell wall. When they were microscopically observed, CjCBM28-fused cyan fluorescent protein (CFP) differentially bound to the root cap, but CjCBM17-fused blue fluorescent protein (BFP) did not. CjCBM17-BFP bound to the central part or the root apical meristem. These results suggest that CjCBM17 and CjCBM28 recognize different sites of the cell wall and that the cell-wall components and the polysaccharides configuration in the cell wall differ between tissues.
来自酒井梭菌的内切葡聚糖酶Cel5A串联包含一个17家族碳水化合物结合模块（CBM）（CjCBM17）和一个28家族CBM（CjCBM28）。这两个CBM与非结晶纤维素和β-1,3-1,4-葡聚糖结合。我们的结果表明，这些CBM识别甘薯根细胞壁上的不同成分。将根切成纵向切片。我们使用与两种不同荧光蛋白融合的CjCBM17和CjCBM28来可视化对植物细胞壁的差异识别。在显微镜下观察时，与CjCBM28融合的青色荧光蛋白（CFP）与根冠有差异结合，但与CjCBM17融合的蓝色荧光蛋白（BFP）则没有。CjCBM17-BFP与根的中部或根尖分生组织结合。这些结果表明，CjCBM17和CjCBM28识别细胞壁的不同位点，并且细胞壁中的细胞壁成分和多糖构型在不同组织之间存在差异。
