Hübner Ulrich, Böckel-Frohnhöfer Nicole, Hummel Björn, Geisel Jürgen
University Hospital of the Saarland, Clinical Chemistry and Laboratory Medicine/Central Laboratory, Homburg, Germany.
Clin Lab. 2010;56(1-2):59-64.
Pneumatic tube sample transport is widely used in hospitals because it is convenient and reduces turnaround times. However, the preanalytical effects of pneumatic tube transport of blood samples on platelet function analysis are not fully understood.
Blood samples from 15 healthy subjects were collected before and after treatment with acetylsalicylic acid. Sample tubes were transported by pneumatic tube transport while the corresponding sample tubes were hand-delivered. Platelet function analysis was performed in platelet rich plasma by optical aggregometry and in whole blood by the PFA-100.
Using the collagen-induced optical aggregometry a significant decrease of the aggregation amplitude (n = 30) was observed in tubed samples in comparison to the corresponding hand-delivered samples (low collagen concentration: 52.5% vs 56.1%, p = 0.006; and high collagen concentration respectively: 63.9% vs 67.1%, p = 0.011). Additionally, a slight prolongation of the PFA-100TM closure time for the epinephrine/collagen and the ADP/collagen stimulation was found in the tubed samples compared to the hand-delivered samples.
The results indicate that the pneumatic tube sample transport impairs the platelet aggregation. Therefore, we recommend the manual transport of whole blood samples which are collected for optical aggregometry or PFA-100 analysis.
气动管道样本传输在医院中广泛应用，因为它便捷且能缩短周转时间。然而，血液样本经气动管道传输对血小板功能分析的分析前影响尚未完全明确。
采集15名健康受试者在服用乙酰水杨酸前后的血样。样本管通过气动管道传输，同时相应的样本管由专人递送。采用光学聚集法在富血小板血浆中以及使用PFA-100在全血中进行血小板功能分析。
使用胶原诱导光学聚集法，与相应的专人递送样本相比，经管道传输的样本中聚集幅度显著降低（n = 30）（低胶原浓度：52.5% 对56.1%，p = 0.006；高胶原浓度分别为：63.9% 对67.1%，p = 0.011）。此外，与专人递送样本相比，经管道传输的样本中肾上腺素/胶原和ADP/胶原刺激的PFA-100TM封闭时间略有延长。
结果表明气动管道样本传输会损害血小板聚集。因此，我们建议对于用于光学聚集法或PFA-100分析而采集的全血样本采用人工传输。
