Departament de Tecnologia Química i de Tensioactius, Institut de Quimica Avançada de Catalunya, Consejo Superior de Investigaciones Científicas, C/Jordi Girona 18-26, 08034 Barcelona, Spain.
Langmuir. 2010 Jul 6;26(13):10578-84. doi: 10.1021/la100691m.
The effect of bicelles formed by dipalmitoylphosphatidylcholine (DPPC)/dihexanoylphosphatidylcholine (DHPC) on stratum corneum (SC) lipids was studied by attenuated total reflectance-Fourier transform infrared (ATR-FTIR) spectroscopy at different temperatures. Analysis of the lipid organization in terms of chain conformational order and lateral packing shows that the use of bicelles hampers the fluidification of SC lipids with temperature and leads to a lateral packing corresponding to a stable hexagonal phase. Grazing incidence small- and wide-angle X-ray scattering (GISAXS and GIWAXS) techniques confirm these results and give evidence of higher lamellar order after treatment with these bicelles. Additionally, the effects of DPPC/DHPC and dimyristoylphosphatidylcholine (DMPC)/DHPC bicelles at different SC depths were compared. The combination of ATR-FTIR spectroscopy and the tape-stripping method was very useful for this purpose.
通过衰减全反射傅里叶变换红外（ATR-FTIR）光谱法在不同温度下研究了由二棕榈酰磷脂酰胆碱（DPPC）/二己酰基磷脂酰胆碱（DHPC）形成的双分子层对角质层（SC）脂质的影响。从链构象有序性和横向堆积的角度分析脂质组织，结果表明，双分子层的使用阻碍了 SC 脂质随温度的流态化，并导致与稳定的六方相相对应的横向堆积。掠入射小角和广角 X 射线散射（GISAXS 和 GIWAXS）技术证实了这些结果，并证明在使用这些双分子层处理后，层状有序性更高。此外，还比较了 DPPC/DHPC 和 DMPC/DHPC 双分子层在不同 SC 深度下的作用。ATR-FTIR 光谱法和胶带剥离法的结合在这方面非常有用。
