Barbosa-Barros L, de la Maza A, Estelrich J, Linares A M, Feliz M, Walther P, Pons R, López O
Departamento de Tecnología de Tensioactivos, Instituto de Investigaciones Químicas y Ambientales de Barcelona, Consejo Superior de Investigaciones Científicas, Calle Jordi Girona 18-26, 08034 Barcelona, Spain.
Langmuir. 2008 Jun 3;24(11):5700-6. doi: 10.1021/la703732h. Epub 2008 May 10.
The effect of dipalmitoyl phosphatidylcholine (DPPC)/dihexanoyl phosphatidylcholine (DHPC) bicelles on the microstructure of pig stratum corneum (SC) in vitro was evaluated. The physicochemical characterization of these nanoaggregates revealed small disks with diameters around 15 nm and a thickness of 5.4 nm. Upon dilution, the bicelles grow and transform into vesicles. Cryogenic scanning electron microscopy (cryo-SEM) images of the SC pieces treated with this system showed vesicles of about 200 nm and lamellar-like structures in the intercellular lipid areas. These vesicles probably resulted from the growth and molecular rearrangement of the DPPC/DHPC bicelles after penetrating the SC. The presence of lamellar-like structures is ascribed to the interaction of the lipids from bicelles with the SC lipids. The bicellar system used is suitable to penetrate the skin SC and to reinforce the intercellular lipid areas, constituting a promising tool for skin applications.
评估了二棕榈酰磷脂酰胆碱（DPPC）/二己酰磷脂酰胆碱（DHPC）双分子层对猪角质层（SC）体外微观结构的影响。这些纳米聚集体的物理化学特征显示为直径约15 nm、厚度为5.4 nm的小圆盘。稀释后，双分子层会生长并转变为囊泡。用该系统处理的SC片的低温扫描电子显微镜（cryo-SEM）图像显示，细胞间脂质区域有直径约200 nm的囊泡和层状结构。这些囊泡可能是DPPC/DHPC双分子层穿透SC后生长和分子重排的结果。层状结构的存在归因于双分子层中的脂质与SC脂质的相互作用。所使用的双分子层系统适用于穿透皮肤SC并加强细胞间脂质区域，是一种有前景的皮肤应用工具。
