AI Virtanen Institute, Department of Molecular Medicine, University of Eastern Finland, FI-70211 Kuopio, Finland.
Curr Gene Ther. 2010 Jun;10(3):187-94. doi: 10.2174/156652310791321206.
Baculoviruses are safe and high-capacity vectors for gene delivery which have matured from the initial successful experiments performed in liver cells into convenient tools to transduce almost any cell from any origin in vitro and ex vivo. This is a result of 15 years of intensive vector development as well as studies performed in vertebrate cells to reveal important factors affecting the transduction efficacy. Now, at the stage when the first evidence of meaningful use of baculoviruses for therapeutic applications has been reported, there is no doubt that the technology will meet the expectations as highly useful platform for many applications of gene delivery. This review summarizes the pre-clinical in vivo work carried out with baculoviruses and discusses remaining challenges which still need to be solved.
杆状病毒是一种安全且大容量的基因传递载体，它已从最初在肝细胞中成功进行的实验成熟为一种便捷的工具，可在体外和体外来转导几乎任何来源的细胞。这是 15 年的载体开发以及在脊椎动物细胞中进行的研究的结果，这些研究揭示了影响转导效率的重要因素。现在，在报道杆状病毒在治疗应用中有意义的首次应用证据的阶段，毫无疑问，该技术将作为一种非常有用的基因传递平台，满足许多应用的期望。本文综述了用杆状病毒进行的临床前体内研究，并讨论了仍需解决的剩余挑战。
