Hu Yu-Chen
Department of Chemical Engineering, National Tsing Hua University Hsinchu, Taiwan 300.
Adv Virus Res. 2006;68:287-320. doi: 10.1016/S0065-3527(06)68008-1.
Since the discovery that baculoviruses can efficiently transduce mammalian cells, baculoviruses have been extensively studied as potential vectors for both in vitro and in vivo gene therapy. This chapter reviews the history of this research area, cells permissive to baculovirus transduction, factors influencing transduction and transgene expression, efforts to improve transduction, mechanisms of virus entry and intracellular trafficking, applications for in vivo and ex vivo gene therapy, as well as advantages, limitations, and safety issues concerning use of baculoviruses as gene therapy vectors. Recent progress and efforts directed toward overcoming existing bottlenecks are emphasized.
自从发现杆状病毒能够有效地转导哺乳动物细胞以来，杆状病毒作为体外和体内基因治疗的潜在载体受到了广泛研究。本章回顾了该研究领域的历史、允许杆状病毒转导的细胞、影响转导和转基因表达的因素、改善转导的努力、病毒进入和细胞内运输的机制、体内和体外基因治疗的应用，以及将杆状病毒用作基因治疗载体的优点、局限性和安全性问题。重点介绍了最近的进展以及为克服现有瓶颈所做的努力。
