Auer D E, Ng J C, Reilly J S, Seawright A A
Department of Veterinary Pathology and Public Health, University of Queensland, St Lucia, Australia.
J Vet Pharmacol Ther. 1991 Mar;14(1):61-9. doi: 10.1111/j.1365-2885.1991.tb00805.x.
The vascular leakage induced by histamine, bradykinin, serotonin and prostaglandin E1 and E2 was assessed. The test agents were injected intradermally into the shaved thoracic skin of horses and the vascular leakage estimated either semi-quantitatively by recording the diameter of the lesions or by measuring the actual volume of extravasated plasma in microliters using iodine-125-labelled human serum albumin (125I-HSA) as a marker in the blood plasma. Using the latter method, the vascular leakage induced by carrageenin and the effect of coadministered prostaglandins E1 and E2 upon the vascular leakage of both histamine and bradykinin were also investigated. No obvious lesions resulted when serotonin (10(-2) mol/l) was injected but histamine and bradykinin produced circular lesions which increased in diameter for approximately 30 min. The size of the lesions and volume of extravasated plasma was dose dependent. On a molar basis, bradykinin (10(-6) mol/l, 10(-5) mol/l) was more potent than histamine but they were equipotent at 10(-4) mol/l. The size of the lesions induced by carrageenin were independent of their anatomical location on the thorax. Except for the second hour, the hourly volume of vascular leakage increased until the fifth hour when the experiment was concluded. The maximum vascular leakage resulting from the injection of prostaglandin E1 or E2 (1, 10, 100 or 1000 ng) was 7 microliters but when co-administered with bradykinin (10(-6) mol/l), the volume of leaked plasma increased from 29 to 78 microliters. No synergy was observed when either prostaglandin was co-administered with histamine (10(-5) mol/l).
评估了组胺、缓激肽、5-羟色胺以及前列腺素E1和E2所诱导的血管渗漏情况。将受试药物皮内注射到马匹剃毛后的胸部皮肤，通过记录损伤直径进行半定量评估血管渗漏，或者使用125I标记的人血清白蛋白（125I-HSA）作为血浆标志物，以微升为单位测量渗出血浆的实际体积来评估血管渗漏。使用后一种方法，还研究了角叉菜胶诱导的血管渗漏以及联合给予前列腺素E1和E2对组胺和缓激肽血管渗漏的影响。注射5-羟色胺（10(-2) mol/l）时未产生明显损伤，但组胺和缓激肽产生圆形损伤，其直径在约30分钟内增大。损伤大小和渗出血浆体积呈剂量依赖性。以摩尔计，缓激肽（10(-6) mol/l，10(-5) mol/l）比组胺更有效，但在10(-4) mol/l时二者效力相当。角叉菜胶诱导的损伤大小与其在胸部的解剖位置无关。除第二小时外，血管渗漏的每小时体积持续增加，直至实验结束的第五小时。注射前列腺素E1或E2（1、10、100或1000 ng）导致的最大血管渗漏为7微升，但与缓激肽（10(-6) mol/l）联合给药时，渗漏血浆体积从29微升增加至78微升。当任何一种前列腺素与组胺（10(-5) mol/l）联合给药时，未观察到协同作用。
