Department of Microbiology, Ajou University School of Medicine, San 5, Wonchon-dong, Yeongtong-gu, Suwon 443-721, Republic of Korea.
Biochem Biophys Res Commun. 2010 May 14;395(4):484-9. doi: 10.1016/j.bbrc.2010.04.032. Epub 2010 Apr 9.
Some proteins with ribonuclease (RNase) activity have been shown to suppress viral replication. A well-characterized recombinant antibody, 3D8 single-chain variable fragment (3D8 scFv), has RNA-hydrolyzing and cell-penetrating activities. Here, we investigated antiviral activity of 3D8 scFv against classical swine fever virus (CSFV). In a cell line expressing 3D8 scFv (C26), intracellular RNA-hydrolysis activity was higher compared to control PK-15 cells and viral replication was strongly suppressed at the viral RNA level, with the evidence of independency of IFN-alpha/beta induction. Exogenous treatment of 3D8 scFv, prior to or post-CSFV infection, was also shown to suppress CSFV replication at the viral RNA level. These observations suggest that antiviral activity of 3D8 scFv may be due to the intrinsic RNase activity of 3D8 scFv, which is capable of targeting viral RNA genomes or transcripts.
一些具有核糖核酸酶（RNase）活性的蛋白质已被证明能抑制病毒复制。一种经过充分研究的重组抗体，3D8 单链可变片段（3D8 scFv），具有 RNA 水解和细胞穿透活性。在这里，我们研究了 3D8 scFv 对猪瘟病毒（CSFV）的抗病毒活性。在表达 3D8 scFv 的细胞系（C26）中，与对照 PK-15 细胞相比，细胞内 RNA 水解活性更高，病毒 RNA 水平上的病毒复制受到强烈抑制，并且 IFN-α/β诱导的独立性证据。在 CSFV 感染之前或之后，用 3D8 scFv 进行外源性处理也显示能抑制 CSFV 复制，达到病毒 RNA 水平。这些观察结果表明，3D8 scFv 的抗病毒活性可能归因于 3D8 scFv 的固有 RNase 活性，该活性能够靶向病毒 RNA 基因组或转录本。
