Oda N, Tahara E, Taniyama K
First Department of Pathology, Hiroshima University School of Medicine, Japan.
Pathol Res Pract. 1989 Apr;184(4):390-401. doi: 10.1016/S0344-0338(89)80034-2.
DNA ploidy patterns in six cases of scirrhous gastric carcinoma were examined by scanning cytophotometry, using 15 micron paraffin sections which were confirmed to involve a single whole nucleus. Measurements of DNA content were performed in 70 to 100 cancer cells each in intramucosal (m), submucosal (sm), propria muscular (pm) and subserosal (ss) parts of the tumor. Out of the six scirrhous gastric carcinomas, four were generalized ones and two were localized ones. Aneuploidy with diploid stem line (mosaic pattern) was seen in two cases (generalized type). The remaining four cases (two cases of localized type and two cases of generalized type) were composed of a diploid stem cell line with or without polyploid cells. Stem cell line did not change through the gastric wall, but in three cases (one diploid case and two mosaic cases), the number of polyploid or aneuploid cells significantly increased at the deep infiltrative site. These findings suggest that DNA ploidy of scirrhous gastric carcinoma is determined at an early stage of tumor proliferation and that the increase of polyploid cells and aneuploid cells in the deeper layer might participate in tumor progression. Moreover, there was no evident correlation between the increase of polyploid or aneuploid cells and the expression of oncogene products or EGF related growth factors.
采用扫描细胞光度术对6例硬癌性胃癌的DNA倍体模式进行了检测，使用的是15微米厚的石蜡切片，证实其中包含单个完整细胞核。分别在肿瘤的黏膜内（m）、黏膜下（sm）、固有肌层（pm）和浆膜下（ss）部分的70至100个癌细胞中进行DNA含量测定。在这6例硬癌性胃癌中，4例为弥漫型，2例为局限型。2例（弥漫型）出现了伴有二倍体干细胞系的非整倍体（镶嵌模式）。其余4例（2例局限型和2例弥漫型）由具有或不具有多倍体细胞的二倍体干细胞系组成。干细胞系在胃壁各层中没有变化，但在3例（1例二倍体病例和2例镶嵌病例）中，多倍体或非整倍体细胞的数量在深层浸润部位显著增加。这些发现表明，硬癌性胃癌的DNA倍体在肿瘤增殖的早期阶段就已确定，更深层中多倍体细胞和非整倍体细胞的增加可能参与了肿瘤进展。此外，多倍体或非整倍体细胞的增加与癌基因产物或表皮生长因子相关生长因子的表达之间没有明显的相关性。
