Tomisaki S, Tatsuo O, Hisao O, Sakaguchi Y, Kusumoto T, Ohno S, Ichiyoshi Y, Maehara Y, Sugimachi K
Dept. of Surgery II, Faculty of Medicine, Kyushu University.
Gan To Kagaku Ryoho. 1995 Jun;22 Suppl 2:115-7.
DNA ploidy pattern was determined by flow cytometry and p53 overexpression was investigated immunohistochemically in 145 primary gastric cancers. Aneuploid patterns were found in 78 (65%) and diploid patterns in 67 (46%). Metastasising to lymph nodes was observed more frequently in cases with aneuploid tumors, as compared to those with diploid tumors (p < 0.01). DNA ploidy patterns were related to overexpression of p53. The incidences of overexpression of p53 in aneuploid tumors and diploid ones were 65% and 33%, respectively (p < 0.05). Thus, we suggest that gastric carcinomas with the aneuploid pattern have mutation of tumor suppressor gene p53 and high potential to metastasize to lymph nodes.
采用流式细胞术测定145例原发性胃癌的DNA倍体模式，并通过免疫组织化学方法研究p53的过表达情况。结果发现，78例（65%）为非整倍体模式，67例（46%）为二倍体模式。与二倍体肿瘤相比，非整倍体肿瘤病例发生淋巴结转移更为常见（p<0.01）。DNA倍体模式与p53的过表达相关。非整倍体肿瘤和二倍体肿瘤中p53过表达的发生率分别为65%和33%（p<0.05）。因此，我们认为具有非整倍体模式的胃癌存在肿瘤抑制基因p53的突变，并且有较高的淋巴结转移潜能。
