结核分枝杆菌H37Rv GroEL1片段的结晶及初步X射线晶体学分析
Crystallization and preliminary X-ray crystallographic analysis of a GroEL1 fragment from Mycobacterium tuberculosis H37Rv.
作者信息
Sielaff Bernhard, Lee Ki Seog, Tsai Francis T F
机构信息
Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston TX 77030, USA.
出版信息
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 Apr 1;66(Pt 4):418-20. doi: 10.1107/S1744309110004409. Epub 2010 Mar 31.
Abstract
Full-length GroEL1 from Mycobacterium tuberculosis H37Rv was cloned, overexpressed and purified. Crystals were obtained by the hanging-drop vapor-diffusion method and contained a 23 kDa GroEL1 fragment. A complete native data set was collected from a single frozen crystal that belonged to the orthorhombic space group P2(1)2(1)2, with unit-cell parameters a = 75.47, b = 78.67, c = 34.89 A, alpha = beta = gamma = 90 degrees , and diffracted to 2.2 A resolution on a home X-ray source.
摘要
克隆、过表达并纯化了来自结核分枝杆菌H37Rv的全长GroEL1。通过悬滴气相扩散法获得晶体，其包含一个23 kDa的GroEL1片段。从属于正交空间群P2(1)2(1)2的单个冷冻晶体收集了完整的原生数据集，晶胞参数为a = 75.47、b = 78.67、c = 34.89 Å，α = β = γ = 90°，并在实验室X射线源上衍射至2.2 Å分辨率。
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