Faculty of Analytical Chemistry, Institute C-1, Department of Chemical Engineering and Technology, Cracow University of Technology, Cracow, Poland.
J Agric Food Chem. 2010 May 12;58(9):5347-54. doi: 10.1021/jf100065w.
Profiles of betacyanins present in light-stressed stems of different cactus species and nonstressed but genetically aberrated red pigmented cacti of Gymnocalycium mihanovichii cv. ;Hibotan' Britton & Rose, which are known as grafted cacti, were compared. The identities of all the pigments in the cacti were characterized for the first time. The identification of acylated and nonacylated betacyanins was performed by means of mass spectrometry and UV-vis diode array detection coupled to high-performance liquid chromatography. The most indicative pigments of the stressed cacti, 5''-O-E-feruloyl-2'-O-beta-apiosyl-betanin and 5''-O-E-sinapoyl-2'-O-beta-apiosyl-betanin, as well as their diasteromers, were the prevailing betacyanins in Hylocereus polyrhizus , Epiphyllum phyllanthus , and Rhipsalis rhombea . Stressed Rhipsalis regnellii stems contained the sinapoylated betacyanins accompanied only by traces of the feruloylated derivatives. In addition, high contents of 2'-O-apiosyl-betanin were frequently observed in the samples with the highest concentration found in stressed Schlumbergera x buckleyi (T. Moore) Tjaden. These pigments were also detected, but at low levels, in the Hibotan pink, red, and violet scions, which were not light-stressed. In the Hibotan scions, the most abundant were the polar betacyanins: betanidin 5-O-beta-sophoroside and betanin. In most of the stressed samples, betanin was present at relatively low levels.
对不同仙人掌物种光胁迫茎和非胁迫但遗传异常的红色色素仙人掌 Gymnocalycium mihanovichii cv. “Hibotan' Britton & Rose 的甜菜红素谱进行了比较，后者被称为嫁接仙人掌。首次对仙人掌中所有色素的身份进行了鉴定。通过质谱和 UV-vis 二极管阵列检测与高效液相色谱联用的方法对酰化和非酰化甜菜红素进行了鉴定。应激仙人掌中最具代表性的色素 5''-O-E-阿魏酰基-2'-O-β-芹糖基甜菜红和 5''-O-E-芥子酰基-2'-O-β-芹糖基甜菜红及其非对映异构体是 Hylocereus polyrhizus、Epiphyllum phyllanthus 和 Rhipsalis rhombea 的主要甜菜红素。应激 Rhipsalis regnellii 茎中含有芥子酰化甜菜红素，仅伴有痕量的阿魏酰基衍生物。此外，在含有最高浓度 2'-O-芹糖基甜菜红的样品中经常观察到高含量的 2'-O-芹糖基甜菜红，在应激 Schlumbergera x buckleyi (T. Moore) Tjaden 中含量最高。这些色素也被检测到，但含量较低，在非光胁迫的 Hibotan 粉色、红色和紫色接穗中含量较低。在 Hibotan 接穗中，最丰富的是极性甜菜红素：甜菜碱 5-O-β-槐糖苷和甜菜红素。在大多数应激样本中，甜菜红素的含量相对较低。
