R C Patel Institute of Pharmaceutical Education and Research, Near Karwand Naka, Shirpur, Dhule District, Maharashtra, India.
Lab Anim. 2010 Jul;44(3):226-30. doi: 10.1258/la.2010.009084. Epub 2010 Apr 12.
The zinc disc implantation-induced urinary bladder calculi model in the rat is commonly used for preclinical evaluation of the antiurolithiatic activity of test compounds. Certain published reports state that relatively long durations for which zinc discs must be implanted in the bladders of rats. Hence, there is a need to refine this model. These investigations aimed to determine whether long-term studies using the zinc disc implantation model provide any additional data that affect the final outcomes of the study. In this study, we evaluated the effects of a well-known antiurolithiatic polyherbal drug, Cystone, for different treatment durations of 10, 20 and 48 days postimplantation. Our results indicate that even the shortest duration of 10 days is sufficient to reveal antiurolithiatic effects of a test drug. Hence, in the zinc disc implantation-induced urinary bladder calculi model, the study duration is proposed to be minimized so as to reduce the distress caused to the rats due to long-term exposure to the implant. Further, it is suggested that the growth of the bladder calculi can be monitored by taking X-ray radiographs of the bladder deposits to decide the time to terminate the study. Use of preformed calcium oxalate crystal instead of zinc discs, as suggested in earlier reports by others, may also be considered to avoid the sacrifice of rats at the end of the study.
锌盘植入诱导的大鼠膀胱结石模型常用于测试化合物抗结石活性的临床前评价。某些已发表的报告指出，锌盘必须在大鼠膀胱中植入相对较长的时间。因此，需要对该模型进行改进。这些研究旨在确定使用锌盘植入模型进行长期研究是否提供了任何影响研究最终结果的额外数据。在这项研究中，我们评估了一种著名的多草药抗结石药物 Cystone 在植入后 10、20 和 48 天不同治疗时间的效果。我们的结果表明，即使是最短的 10 天治疗时间也足以显示测试药物的抗结石作用。因此，在锌盘植入诱导的膀胱结石模型中，建议将研究时间缩短到最低限度，以减少由于长期暴露于植入物而对大鼠造成的痛苦。此外，建议通过对膀胱沉积物进行 X 射线射线照相来监测膀胱结石的生长，以决定终止研究的时间。如其他人先前的报告所建议，使用预制的草酸钙晶体代替锌盘，也可以考虑避免在研究结束时处死大鼠。
