Laboratory for Biologically Oriented Materials, Department of Materials, ETH Zurich, Zurich, Switzerland.
Nano Lett. 2010 May 12;10(5):1823-30. doi: 10.1021/nl100533c.
Quantifying cellular forces relies on accurate calibrations of the sensor stiffness. Neglecting deformations of elastic substrates to which elastic pillars are anchored systematically overestimates the applied forces (up to 40%). A correction factor considering substrate warping is derived analytically and verified experimentally. The factor scales with the dimensionless pillar aspect ratio. This has significant implications when designing pillar arrays or comparing absolute forces measured on different pillar geometries during cell spreading, motility, or rigidity sensing.
量化细胞力依赖于传感器刚度的精确校准。如果忽略与弹性支柱相连的弹性基底的变形,那么系统地会高估施加的力(高达 40%)。从理论上推导出了一个考虑基底翘曲的修正因子,并通过实验进行了验证。该因子与无因次支柱纵横比成正比。当设计支柱阵列或在细胞扩展、迁移或刚性感测期间比较不同支柱几何形状上测量的绝对力时,这具有重要意义。