Division of Allergy, Immunology and Rheumatology, Taichung Veterans General Hospital, Taichung, Taiwan, ROC.
Connect Tissue Res. 2010 Oct;51(5):370-7. doi: 10.3109/03008200903461462.
To determine circulating levels of CD4(+)CD25(high) regulatory T (Treg) cells and transforming growth factor-β (TGF-β) in patients with adult-onset Still's disease (AOSD) and to examine the associations with disease activity and clinical course of this disease.
The frequencies of circulating CD4(+)CD25(high) Treg cells in 52 active AOSD patients, 42 active systemic lupus erythematosus (SLE) patients, and 22 healthy controls (HCs) were determined using flow cytometry analysis. Levels of serum TGF-β and soluble interleukin-2 receptor (sIL-2R) were measured by enzyme-linked immunosorbent assay.
Significantly lower levels of circulating CD4(+)CD25(high) Treg cells and serum TGF-β were found in AOSD patients and SLE patients than those found in HCs. Levels of circulating CD4(+)CD25(high) Treg cells and TGF-β were inversely correlated with disease activity scores for AOSD patients and SLE patients. Circulating CD4(+)CD25(high) Treg cell frequencies were positively correlated with serum TGF-β levels for patients with both diseases. Levels of circulating CD4(+)CD25(high) Treg cells and TGF-β significantly increased, paralleling clinical remission and the decrease in levels of C-reactive protein and soluble interleukin-2 receptor after effective therapy in AOSD patients. AOSD patients with monocyclic course had significantly higher levels of circulating CD4(+)CD25(high) Treg cells and TGF-β compared to those with polycyclic and chronic articular course.
Diminished levels of circulating CD4(+)CD25(high) Treg cells and TGF-β, and inverse correlation with disease activity in patients with AOSD and SLE might be involved in the pathogenesis of both diseases. Increased levels of circulating CD4(+)CD25(high) Treg cells or TGF-β might be associated with a favorable clinical course in AOSD patients.
测定成人Still 病（AOSD）患者外周血 CD4+CD25+高调节性 T（Treg）细胞和转化生长因子-β（TGF-β）的水平，并探讨其与疾病活动度及临床病程的关系。
采用流式细胞术检测 52 例活动期 AOSD 患者、42 例活动期系统性红斑狼疮（SLE）患者及 22 例健康对照者外周血 CD4+CD25+Treg 细胞的频率，酶联免疫吸附试验（ELISA）法检测血清 TGF-β及可溶性白细胞介素-2 受体（sIL-2R）水平。
AOSD 患者和 SLE 患者外周血 CD4+CD25+Treg 细胞及血清 TGF-β水平均显著低于健康对照组。AOSD 患者和 SLE 患者外周血 CD4+CD25+Treg 细胞及 TGF-β水平与疾病活动评分呈负相关。两病患者外周血 CD4+CD25+Treg 细胞频率与血清 TGF-β水平呈正相关。经有效治疗后 AOSD 患者临床缓解及 C 反应蛋白、sIL-2R 水平下降时，外周血 CD4+CD25+Treg 细胞及 TGF-β水平显著升高。与多关节型及慢性关节型病程比较，单关节型 AOSD 患者外周血 CD4+CD25+Treg 细胞及 TGF-β水平显著升高。
AOSD 与 SLE 患者外周血 CD4+CD25+Treg 细胞及 TGF-β水平降低，与疾病活动度呈负相关，可能参与了两病的发病机制。AOSD 患者外周血 CD4+CD25+Treg 细胞或 TGF-β水平升高可能与临床转归良好有关。
