Ajdary Soheila, Ghamilouie Mir-Mostafa, Alimohammadian Mohammad Hossein, Hosseini Mansoureh, Pakzad Saeed-Reza
Immunology Department, Pasteur Institute of Iran, Tehran, Islamic Republic of Iran.
Parasitol Int. 2010 Sep;59(3):466-8. doi: 10.1016/j.parint.2010.03.008. Epub 2010 Apr 11.
The aim of the present study was to investigate the frequency of Arg753Gln and Arg677Trp polymorphisms of TLR2 in patients with cutaneous leishmaniasis (CL) compared to healthy controls. The polymorphisms were genotyped by polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) and amplification refractory mutation system assay (ARMS-PCR). The results showed that the frequency of Arg753Gln genotype was 14.3% and 10.1% in CL patients and normal controls, respectively. No one in either group was homozygous for the mutation. There was no significant difference in the genotype frequency. In contrast to the results for Arg753Gln polymorphism, we did not detect any case with Arg677Trp polymorphism in either control or patient group. In conclusion the TLR2 mutations are found equally in CL patients and healthy subjects.
本研究的目的是调查与健康对照相比，皮肤利什曼病（CL）患者中TLR2基因的Arg753Gln和Arg677Trp多态性的频率。通过聚合酶链反应-限制性片段长度多态性（PCR-RFLP）和扩增阻滞突变系统分析（ARMS-PCR）对多态性进行基因分型。结果显示，CL患者和正常对照中Arg753Gln基因型的频率分别为14.3%和10.1%。两组中均无人为该突变的纯合子。基因型频率无显著差异。与Arg753Gln多态性的结果相反，在对照组或患者组中我们均未检测到任何携带Arg677Trp多态性的病例。总之，在CL患者和健康受试者中发现TLR2突变的情况相同。
