[促肾上腺皮质激素对神经发生早期大鼠胚胎脑内核酸与蛋白质合成及含量的影响]
[Effect of adrenocorticotropic hormone on nucleic acid and protein synthesis and content in the brain of rat embryos in the early stages of neurogenesis].
作者信息
Kotin A M, Chebotar' N A, Tishchenko L I
出版信息
Ontogenez. 1978;9(1):70-7.
The injection of adrenocorticotropic hormone (ACTH) of prolonged effect at the doses of 4 and 10 M. U. to the intact rats from the 11th till the 15th day of pregnancy resulted in the twofold increase of protein content in the brain and its decrease in the liver of 15 days old embryos, as compared with the control ones. The content of DNA, RNA and proteins in the placenta of experimental animals increased as well. The rate of incorporation of 3H-thymidine in the liver DNA and 14C-leucine in the liver and brain acid-soluble protein decreased within small intervals of time following the treatment. The total radioactivity of proteins in the liver, brain and placenta calculated per DNA unit was similar to the control one whereas the specific radioactivity of total protein in the liver of experimental embryos was higher than in the control.
在妊娠第11天至第15天，对未受损伤的大鼠注射剂量为4和10 M.U.的长效促肾上腺皮质激素（ACTH），与对照组相比，15日龄胚胎的脑蛋白含量增加了两倍，而肝脏中的蛋白含量则下降。实验动物胎盘内的DNA、RNA和蛋白质含量也有所增加。在治疗后的短时间内，肝脏DNA中3H-胸腺嘧啶核苷的掺入率以及肝脏和脑酸溶性蛋白中14C-亮氨酸的掺入率均下降。以每个DNA单位计算，肝脏、脑和胎盘内蛋白质的总放射性与对照组相似，而实验胚胎肝脏中总蛋白的比放射性高于对照组。
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