Jakoubek B, Hájek I
Physiol Bohemoslov. 1976;25(4):301-7.
In 2-day-old rats, ACTH increases the uptake of 3H-uridine in the brain; no such effects were observed in the msucle and liver tissue. Diazepam does not affect the uptake of 3H-uridine into the brain tissue, but decreases the uptake into the muscle tissue. In the brain tissue of infant rats, ACTH does not change the distribution of radioactivity of intraperitoneally administered 3H-uridine among U, Urd, UMP, UDP and UTP. Diazepam increases the amount of radioactivity, found at the U spot. In the muscle, both ACTH and Diazepam increases the amount of radioactivities found in the Urd spot. A significant correlation between the radioactivity of UMP+UDP+UTP and the specific activity of RNA was proved only in the brain. The ratio: specific activity of brain RNA/radioactivity of UMP+UDP+UTP was thus used as a tentative indicator of the biosynthesis of brain RNA. Neither ACTH nor diazepam altered this ratio in infant rats.
在2日龄大鼠中，促肾上腺皮质激素（ACTH）可增加大脑对³H - 尿苷的摄取；在肌肉和肝脏组织中未观察到此类作用。地西泮不影响³H - 尿苷进入脑组织的摄取，但会降低其进入肌肉组织的摄取。在幼鼠的脑组织中，ACTH不会改变腹腔注射的³H - 尿苷在尿苷（U）、尿嘧啶核苷（Urd）、尿苷一磷酸（UMP）、尿苷二磷酸（UDP）和尿苷三磷酸（UTP）之间的放射性分布。地西泮会增加在U点发现的放射性量。在肌肉中，ACTH和地西泮都会增加在Urd点发现的放射性量。仅在大脑中证明了UMP + UDP + UTP的放射性与RNA的比活性之间存在显著相关性。因此，大脑RNA比活性/UMP + UDP + UTP放射性的比值被用作大脑RNA生物合成的初步指标。在幼鼠中，ACTH和地西泮均未改变该比值。
