Anandjiwala Sheetal, Bagul M S, Parabia M, Rajani M
Pharmacognosy and Phytochemistry Department, B. V. Patel Pharmaceutical Education Research Development (PERD) Centre, Thaltej, Ahemdabad - 380 054, India.
Indian J Pharm Sci. 2008 Jan;70(1):31-5. doi: 10.4103/0250-474X.40328.
We report the free radical scavenging activity of an Ayurvedic preparation Panchvalkala and its individual components (stem bark of Ficus benghalensis, F. glomerata, F. religiosa, F. virens and Thespesia populnea). Being stem barks, these samples contain phenolics (ranging from 3.5% to 10.8% w/w) and tannins (1.6% to 7.0% w/w). This prompted us to study the free radical scavenging activity of Panchvalkala and its components which was evaluated in three in vitro models viz. 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity, superoxide radical scavenging activity and reducing power assay. Panchvalkala and its individual components showed significant antiradical activity by bleaching 1,1-diphenyl-2-picrylhydrazyl radical (EC(50) ranging from 7.27 to 12.08 microg) which was comparable to pyrogallol (EC(50) 4.85 microg). Thin layer chromatography of the methanol extracts when sprayed with 0.2% 1,1-diphenyl-2-picrylhydrazyl in methanol revealed several bands with antiradical activity as seen by bleaching of 1,1-diphenyl-2-picrylhydrazyl. All the samples showed good superoxide scavenging potential (EC(50) ranging from 41.55 to 73.56 microg) comparable to ascorbic acid (EC(50) 45.39 microg) in a dose-dependent manner. The reduction ability, Fe(3+) to Fe(2+) transformation was found to increase with increasing concentrations of all the sample extracts.
我们报告了一种阿育吠陀制剂五叶卡勒及其各个成分（孟加拉榕、聚果榕、菩提树、琴叶榕和杨叶肖槿的茎皮）的自由基清除活性。作为茎皮，这些样品含有酚类物质（含量为3.5%至10.8% w/w）和单宁（1.6%至7.0% w/w）。这促使我们研究五叶卡勒及其成分的自由基清除活性，该活性在三种体外模型中进行了评估，即1,1-二苯基-2-苦基肼自由基清除活性、超氧阴离子自由基清除活性和还原能力测定。五叶卡勒及其各个成分通过使1,1-二苯基-2-苦基肼自由基褪色显示出显著的抗自由基活性（半数有效浓度（EC(50)）范围为7.27至12.08微克），这与连苯三酚（EC(50) 4.85微克）相当。当用0.2%的1,1-二苯基-2-苦基肼甲醇溶液喷洒甲醇提取物的薄层色谱时，如1,1-二苯基-2-苦基肼褪色所示，显示出几条具有抗自由基活性的条带。所有样品均显示出良好的超氧阴离子清除潜力（EC(50)范围为41.55至73.56微克），与抗坏血酸（EC(50) 45.39微克）相当，呈剂量依赖性。发现随着所有样品提取物浓度的增加，还原能力（Fe(3+)向Fe(2+)的转化）也会增加。
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