Samarakoon S M S, Chandola H M, Shukla V J
Department of Kayachikitsa, GWAI, University of Kelaniya, Sri Lanka, India .
Int J Ayurveda Res. 2011 Jan;2(1):23-8. doi: 10.4103/0974-7788.83186.
Amalakayas Rasayana (AR) is a polyherbal formulation mentioned in Ayurveda to treat aging and age-associated diseases. Being an antiaging drug, AR may have antioxidants and free radical scavenging activity to minimize free radical-induced damage which is a key cause of aging. The methanolic extract of AR was evaluated in vitro for total phenolic and tannin content, free radical scavenging activity, superoxide radical scavenging activity, and reducing power. The total phenolic content was measured using Folin-ciocalteu reagent against gallic acid [relative standard deviation (R(2)) = 0.998]. Total tannin was estimated using the Stephen method and was found to be 2.82% w/w. Free radical scavenging activity was measured by 2,2-diphenyl-1-picryl hydrazyl assay and R(2) was 1. Superoxide radical scavenging activity was done by ethylene diamine tetra acetate and Nitro Blue Tetrazolium Chloride assays against ascorbic acid and R(2) was 0.976 (EC(50)= 77.5 μg/ml). Ferrous reducing power was evaluated by Oyaizu method where R(2) was 0.986. All studies showed that AR possesses antioxidant activity. The results of this study suggest that the antioxidant and free radical scavenging activity of AR may explain its rasayana effect and justify its use as a medicine for age associated diseases.
阿玛拉卡亚斯·拉萨亚纳（AR）是阿育吠陀中提到的一种多草药配方，用于治疗衰老及与年龄相关的疾病。作为一种抗衰老药物，AR可能具有抗氧化剂和自由基清除活性，以将自由基诱导的损伤降至最低，而自由基诱导的损伤是衰老的关键原因。对AR的甲醇提取物进行了体外总酚和单宁含量、自由基清除活性、超氧阴离子自由基清除活性及还原能力的评估。使用福林-西奥尔特试剂针对没食子酸测定总酚含量[相对标准偏差（R(2)）= 0.998]。采用斯蒂芬法估算总单宁，发现其含量为2.82% w/w。通过2,2-二苯基-1-苦基肼法测定自由基清除活性，R(2)为1。通过乙二胺四乙酸和氯化硝基四氮唑蓝法针对抗坏血酸进行超氧阴离子自由基清除活性测定，R(2)为0.976（半数有效浓度（EC(50)）= 77.5 μg/ml）。采用大津法评估亚铁还原能力，R(2)为0.986。所有研究均表明AR具有抗氧化活性。本研究结果表明，AR的抗氧化和自由基清除活性可能解释其拉萨亚纳效应，并证明其作为治疗与年龄相关疾病药物的合理性。
