Department of Microbiology and Immunology, Biosciences Institute, UNESP, Botucatu, SP, Brazil.
J Ethnopharmacol. 2010 Jun 16;129(3):314-8. doi: 10.1016/j.jep.2010.03.030. Epub 2010 Apr 12.
Propolis is a natural product extensively used in food and beverages to improve health and to prevent diseases, showing immunomodulatory properties. The goal of this work was to evaluate the effect of propolis administration over a short-term to mice on Th1 (IL-2 and IFN-gamma) and Th2 (IL-4 and IL-10) cytokines' expression and production.
Propolis was administered for 3 days to mice by gavage, spleens were removed and RNA was extracted to assess cytokines' expression by real-time PCR. Supernatants of spleen cell cultures were used for cytokines determination by ELISA.
Propolis administration to mice did not affect IL-2, IL-4 and IL-10 expression and production, while IFN-gamma production was inhibited in the splenocyte cultures stimulated or not by Con A.
Since IFN-gamma is a pro-inflammatory cytokine, our data suggest that propolis administration over a short-term to mice may be associated with anti-inflammatory effects in vivo, and further assays could check propolis efficiency in inflammatory diseases.
蜂胶是一种广泛应用于食品和饮料中的天然产物，具有改善健康和预防疾病的作用，同时表现出免疫调节特性。本研究的目的是评估短期给予蜂胶对小鼠 Th1（IL-2 和 IFN-γ）和 Th2（IL-4 和 IL-10）细胞因子表达和产生的影响。
通过灌胃给予蜂胶 3 天，取出脾脏并提取 RNA 以通过实时 PCR 评估细胞因子的表达。使用 ELISA 测定脾细胞培养物上清液中的细胞因子。
蜂胶给予小鼠后不影响 IL-2、IL-4 和 IL-10 的表达和产生，而 Con A 刺激或不刺激的脾细胞培养物中 IFN-γ 的产生受到抑制。
由于 IFN-γ 是一种促炎细胞因子，我们的数据表明，短期给予小鼠蜂胶可能与体内抗炎作用有关，进一步的研究可以检测蜂胶在炎症性疾病中的功效。
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