Department of Microbiology and Immunology, Biosciences Institute, UNESP, Campus of Botucatu 18618-000, Brazil.
Phytother Res. 2010 Oct;24(10):1501-7. doi: 10.1002/ptr.3142.
Since propolis possesses immunomodulatory and antitumoral activities, this work aimed to evaluate its effect on Th1 (IL-2 and IFN-γ) and Th2 (IL-4 and IL-10) cytokines mRNA expression and production by melanoma-bearing mice submitted to immobilization stress. C57BL/6 male mice were inoculated with B16F10 cells, treated with propolis and submitted to stress for 14 days. Spleen cells were assessed for Th1/Th2 cytokine expression and production. Stress induced a higher tumor area, while propolis-treated mice, stressed or not, showed a melanoma development similar to the control. In groups without melanoma, stress or propolis treatment did not affect IL-2, IL-4 and IL-10 gene expression. On the other hand, IL-2 and IL-10 expression was inhibited in melanoma-bearing mice, stressed or not. Th1 cytokine production was also inhibited in melanoma-bearing mice. Propolis administration to melanoma-bearing mice submitted to stress stimulated IL-2 expression, as well as Th1 cytokine (IL-2 and IFN-γ) production, indicating the activation of antitumor cell-mediated immunity. Propolis also stimulated IL-10 expression and production, which may be related to immunoregulatory effects. The data indicate that propolis exerted an immunomodulatory activity in this assay, which may be related to its antitumoral action in vivo.
由于蜂胶具有免疫调节和抗肿瘤活性，本工作旨在评估其对荷黑色素瘤小鼠 Th1（IL-2 和 IFN-γ）和 Th2（IL-4 和 IL-10）细胞因子 mRNA 表达和产生的影响，这些小鼠经历了固定应激。将 C57BL/6 雄性小鼠接种 B16F10 细胞，用蜂胶处理，并进行 14 天的应激。评估脾细胞中 Th1/Th2 细胞因子的表达和产生。应激诱导更高的肿瘤面积，而无论是否应激，用蜂胶处理的荷瘤小鼠表现出与对照相似的黑色素瘤发展。在没有黑色素瘤的组中，应激或蜂胶处理均不影响 IL-2、IL-4 和 IL-10 基因表达。另一方面，IL-2 和 IL-10 的表达在应激或不应激的荷瘤小鼠中受到抑制。Th1 细胞因子的产生也在荷瘤小鼠中受到抑制。给应激荷瘤小鼠施用蜂胶刺激了 IL-2 的表达，以及 Th1 细胞因子（IL-2 和 IFN-γ）的产生，表明抗肿瘤细胞介导的免疫被激活。蜂胶还刺激了 IL-10 的表达和产生，这可能与免疫调节作用有关。数据表明，蜂胶在该实验中发挥了免疫调节作用，这可能与其体内的抗肿瘤作用有关。
