多壁碳纳米管的原位沉积羟基磷灰石功能化。
The functionalization of multi-walled carbon nanotubes by in situ deposition of hydroxyapatite.
机构信息
School of Materials Science and Engineering, Key Laboratory of Advanced Technologies of Materials, Ministry of Education, Southwest Jiaotong University, Chengdu 610031, Sichuan, PR China.
出版信息
Biomaterials. 2010 Jul;31(19):5182-90. doi: 10.1016/j.biomaterials.2010.03.012. Epub 2010 Apr 13.
A simple and effective approach was introduced to functionalize multi-walled carbon nanotubes (MWNTs) by in situ deposition of hydroxyapatite (HA) to improve their hydrophilicity and biocompatibility. Firstly, we prepared two types of pre-functionalized MWNTs: acid-oxidated MWNTs and covalently modified MWNTs by poly (ethylene glycol) (PEG). The influences of the acid-oxidated time, pre-phosphorylation, and PEGylation of MWNTs on in situ growth of HA were further investigated in simulated body fluid (SBF) with ionic concentration: 2, 5 and 10 times, respectively, at 37 degrees C for 24h. The results exhibited that all these factors have positive effects on the HA crystals growth, especially the PEGylation of MWNTs plays a key role during the deposition. Finally, the methyl thiazolyl tetrazolium (MTT) assay was performed to evaluate their cytotoxicity, which showed that the PEGylated MWNTs wrapped by HA crystals have the best biocompatibility.
本研究提出了一种简单有效的方法，通过原位沉积羟基磷灰石（HA）对多壁碳纳米管（MWNTs）进行功能化，以提高其亲水性和生物相容性。首先，我们制备了两种类型的预功能化 MWNTs：酸氧化 MWNTs 和通过聚乙二醇（PEG）共价修饰的 MWNTs。进一步研究了酸氧化时间、MWNTs 的预磷酸化和 PEG 化对在模拟体液（SBF）中离子浓度为 2、5 和 10 倍，37°C 条件下 24 小时内原位生长 HA 的影响。结果表明，所有这些因素都对 HA 晶体的生长有积极影响，特别是 MWNTs 的 PEG 化在沉积过程中起着关键作用。最后，通过噻唑蓝（MTT）法评估了它们的细胞毒性，结果表明被 HA 晶体包裹的 PEG 化 MWNTs 具有最佳的生物相容性。
Zotero 插件