Daavari Morteza, Atapour Masoud, Mohedano Marta, Matykina Endzhe, Arrabal Raul, Nesic Dobrila
Department of Materials Engineering, Isfahan University of Technology, Isfahan 84156-83111, Iran.
Departamento de Ingeniería Química y de Materiales, Facultad de Ciencias Químicas, Universidad Complutense, 28040 Madrid, Spain.
J Funct Biomater. 2023 Sep 16;14(9):475. doi: 10.3390/jfb14090475.
To regulate the degradation rate and improve the surface biocompatibility of the AZ31B magnesium alloy, three different coating systems were produced via plasma electrolytic oxidation (PEO): simple PEO, PEO incorporating multi-walled carbon nanotubes (PEO + CNT), and a duplex coating that included a polycaprolactone top layer (PEO + CNT/PCL). Surfaces were characterized by chemical content, roughness, topography, and wettability. Biological properties analysis included cell metabolism and adhesion. PEO ± CNT resulted in an augmented surface roughness compared with the base material (BM), while PCL deposition produced the smoothest surface. All surfaces had a contact angle below 90°. The exposure of gFib-TERT and bmMSC to culture media collected after 3 or 24 h did not affect their metabolism. A decrease in metabolic activity of 9% and 14% for bmMSC and of 14% and 29% for gFib-TERT was observed after 3 and 7 days, respectively. All cells died after 7 days of exposure to BM and after 15 days of exposure to coated surfaces. Saos-2 and gFib-TERT adhered poorly to BM, in contrast to bmMSC. All cells on PEO anchored into the pores with filopodia, exhibited tiny adhesion protrusions on PEO + CNT, and presented a web-like spreading with lamellipodia on PEO + CNT/PCL. The smooth and homogenous surface of the duplex PEO + CNT/PCL coating decreased magnesium corrosion and led to better biological functionality.
为了调节AZ31B镁合金的降解速率并改善其表面生物相容性，通过等离子体电解氧化（PEO）制备了三种不同的涂层体系：单纯PEO、含多壁碳纳米管的PEO（PEO + CNT）以及包含聚己内酯顶层的双层涂层（PEO + CNT/PCL）。通过化学成分、粗糙度、形貌和润湿性对表面进行了表征。生物学性能分析包括细胞代谢和黏附。与基材（BM）相比，PEO ± CNT使表面粗糙度增加，而PCL沉积产生了最光滑的表面。所有表面的接触角均低于90°。将gFib-TERT和bmMSC暴露于3小时或24小时后收集的培养基中，并未影响它们的代谢。分别在3天和7天后观察到bmMSC的代谢活性下降了9%和14%，gFib-TERT的代谢活性下降了14%和29%。暴露于BM 7天后以及暴露于涂层表面15天后，所有细胞均死亡。与bmMSC相比，Saos-2和gFib-TERT在BM上的黏附较差。PEO上的所有细胞都通过丝状伪足锚定在孔中，在PEO + CNT上表现出微小的黏附突起，在PEO + CNT/PCL上呈现出带有片状伪足的网状铺展。双层PEO + CNT/PCL涂层光滑均匀的表面降低了镁的腐蚀并导致了更好的生物学功能。
