Department of Gastrointestinal Sciences, Christian Medical College, Vellore 632004, India.
J Clin Microbiol. 2010 Jun;48(6):2075-81. doi: 10.1128/JCM.02509-09. Epub 2010 Apr 14.
Cryptosporidium spp., a common cause of diarrhea in children, were investigated in the first multisite study in India. Diarrheal stools from hospitalized children aged <5 years from Delhi, Trichy, and Vellore were analyzed by microscopy, PCR-restriction fragment length polymorphism (RFLP), and/or sequencing at the small-subunit (SSU) rRNA and Cpgp40/15 loci for species determination and subgenotyping, respectively. Seventy of 2,579 (2.7%) children, 75% of whom were <2 years old, had cryptosporidial diarrhea as determined by microscopy. Genotyping and subgenotyping showed that Cryptosporidium hominis was the most commonly identified species (59/67 children), and subgenotypes Ie, Ia, Ib, and Id were common in all centers. A novel C. parvum subgenotype, IIn, was identified in Vellore. Meteorological analysis revealed a higher rate of cryptosporidial positivity during hotter and drier weather in Delhi.
在印度进行的首次多地点研究中,调查了导致儿童腹泻的常见病原体——隐孢子虫。对来自德里、特里奇和韦洛尔的住院年龄<5 岁的儿童的腹泻粪便进行显微镜检查、PCR-限制性片段长度多态性(RFLP)和/或测序,以确定小亚基(SSU)rRNA 和 Cpgp40/15 基因座的种属和亚基因型。在 2579 名儿童中,有 70 名(2.7%)被确认为显微镜下的隐孢子虫腹泻。基因分型和亚基因分型表明,隐孢子虫虫是最常见的鉴定物种(67 名儿童中的 59 名),所有中心均常见 Ie、Ia、Ib 和 Id 亚基因型。在韦洛尔发现了一种新的隐孢子虫 C. parvum 亚基因型 IIn。气象分析显示,在德里天气炎热干燥时,隐孢子虫阳性率更高。