Leoni F, Amar C, Nichols G, Pedraza-Díaz S, McLauchlin J
Health Protection Agency Centre for Infections, 61 Colindale Avenue, London NW9 5EQ, UK.
J Med Microbiol. 2006 Jun;55(Pt 6):703-707. doi: 10.1099/jmm.0.46251-0.
The characterization of Cryptosporidium using DNA extracted from whole faecal samples collected from 2414 humans with diarrhoea in England between 1985 and 2000 where cryptosporidial oocysts were detected using conventional methods is described. Characterization was achieved by PCR/RFLP and DNA sequencing of fragments of the Cryptosporidium oocyst wall protein and the 18S rDNA genes. Cryptosporidium parvum was detected in 56.1 % of cases, Cryptosporidium hominis in 41.7 % and a mixture of C. parvum and C. hominis in 0.9 %. In the remainder of cases, Cryptosporidium meleagridis (0.9 %), Cryptosporidium felis (0.2 %), Cryptosporidium andersoni (0.1 %), Cryptosporidium canis (0.04 %), Cryptosporidium suis (0.04 %) and the Cryptosporidium cervine type (0.04 %) were detected.
本文描述了对1985年至2000年间在英格兰收集的2414例腹泻患者的全粪便样本中提取的DNA进行隐孢子虫特征分析的情况，这些样本是采用传统方法检测到隐孢子虫卵囊的。通过对隐孢子虫卵囊壁蛋白和18S rDNA基因片段进行PCR/RFLP和DNA测序来实现特征分析。在56.1%的病例中检测到微小隐孢子虫，41.7%检测到人隐孢子虫，0.9%检测到微小隐孢子虫和人隐孢子虫的混合感染。在其余病例中，检测到火鸡隐孢子虫（0.9%）、猫隐孢子虫（0.2%）、安氏隐孢子虫（0.1%）、犬隐孢子虫（0.04%）、猪隐孢子虫（0.04%）和鹿隐孢子虫型（0.04%）。
