Mistry S C, Priestman D A, Kerbey A L, Randle P J
Nuffield Department of Clinical Biochemistry, John Radcliffe Hospital, Oxford, U.K.
Biochem J. 1991 May 1;275 ( Pt 3)(Pt 3):775-9. doi: 10.1042/bj2750775.
It is shown here that rat liver pyruvate dehydrogenase (PDH) kinase activator protein (KAP) catalyses ATP-dependent inactivation and [32P]phosphorylation of pig heart PDHE1 and of yeast (Saccharomyces cerevisiae) PDH complex devoid of PDH kinase activity, that fluorosulphonylbenzoyladenosine inactivates rat liver KAP and the intrinsic PDH kinase of rat liver PDH complex, and that KAP, like PDH kinase, is inactivated by thiol-reactive reagents. It is concluded that KAP is a free PDH kinase.
本文表明,大鼠肝脏丙酮酸脱氢酶(PDH)激酶激活蛋白(KAP)催化猪心脏PDHE1和缺乏PDH激酶活性的酵母(酿酒酵母)PDH复合物的ATP依赖性失活和[32P]磷酸化,氟磺酰苯甲酰腺苷使大鼠肝脏KAP和大鼠肝脏PDH复合物的内在PDH激酶失活,并且KAP与PDH激酶一样,可被硫醇反应性试剂失活。得出的结论是,KAP是一种游离的PDH激酶。