胰岛素可逆转饥饿对培养肝细胞中丙酮酸脱氢酶激酶活性的影响。
Insulin reverses effects of starvation on the activity of pyruvate dehydrogenase kinase in cultured hepatocytes.
作者信息
Marchington D R, Kerbey A L, Jones A E, Randle P J
机构信息
Nuffield Department of Clinical Biochemistry, University of Oxford, John Radcliffe Hospital, U.K.
出版信息
Biochem J. 1987 Aug 15;246(1):233-6. doi: 10.1042/bj2460233.
Abstract
In tissue culture of hepatocytes, insulin (0.1-1 munits/ml for 4 h) reversed completely the effects of starvation of rats to decrease the activity of pyruvate dehydrogenase (PDH) complex and to increase the activities of PDH kinase and PDH kinase activator protein. It had no effect in hepatocytes from fed rats. Significant effects of insulin were detected with 0.01 munit/ml after 4 h, and in 1-2 h with 1 munit/ml.
摘要
在肝细胞的组织培养中，胰岛素（0.1 - 1微单位/毫升，作用4小时）完全逆转了饥饿大鼠丙酮酸脱氢酶（PDH）复合体活性降低以及PDH激酶和PDH激酶激活蛋白活性增加的效应。对喂食大鼠的肝细胞则无此作用。4小时后，0.01微单位/毫升的胰岛素可检测到显著效应，1微单位/毫升的胰岛素在1 - 2小时即可检测到显著效应。
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