肿瘤坏死因子-α（TNFα）基因多态性与急性胰腺炎。
Polymorphisms in tumour necrosis factor alpha (TNFalpha) gene in patients with acute pancreatitis.
机构信息
Department of Pharmaceutical Toxicology, Faculty of Pharmacy, Istanbul University, 34116 Istanbul, Turkey.
出版信息
Mediators Inflamm. 2010;2010:482950. doi: 10.1155/2010/482950. Epub 2010 Apr 14.
Proinflammatory cytokines, such as tumour necrosis factor alpha (TNFalpha), play fundamental roles in the pathogenesis of acute pancreatitis (AP). The aim of this study was to determine if polymorphisms in the TNFalpha gene are associated with AP. Two polymorphisms located in the promoter region (positions -308 and -238) in TNFalpha gene were determined using polymerase chain reaction- (PCR-) restriction fragment length polymorphism (RFLP) methods in 103 patients with AP and 92 healthy controls. Odds ratios (ORs) and 95% confidence intervals (CI) were estimated using logistic regression analysis adjusted for age, sex, BMI and smoking. The frequencies of TNFalpha polymorphisms were both similar in patients with mild or severe pancreatitis, so were in pancreatitis patients and in controls. We suggest that both SNPs of TNFalpha are not genetic risk factor for AP susceptibility (OR = 1.63; 95% CI: 1.13-4.01 for TNFalpha(-308) and OR = 0.86; 95% CI: 0.75-1.77 for TNFalpha(-238)).
促炎细胞因子，如肿瘤坏死因子 α（TNFalpha），在急性胰腺炎（AP）的发病机制中起重要作用。本研究旨在确定 TNFalpha 基因中的多态性是否与 AP 相关。使用聚合酶链反应 - （PCR）- 限制性片段长度多态性（RFLP）方法在 103 例 AP 患者和 92 例健康对照中确定 TNFalpha 基因启动子区域（位置 -308 和 -238）的两个多态性。使用 logistic 回归分析调整年龄、性别、BMI 和吸烟因素后，估计比值比（OR）和 95%置信区间（CI）。TNFalpha 多态性的频率在轻度或重度胰腺炎患者、胰腺炎患者和对照组中均相似。我们认为 TNFalpha 的这两个 SNP 都不是 AP 易感性的遗传风险因素（TNFalpha(-308)的 OR = 1.63；95%CI：1.13-4.01，TNFalpha(-238)的 OR = 0.86；95%CI：0.75-1.77）。
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