通过种子乳液聚合制备的生物功能镧系元素标记聚合物颗粒及其通过新型电感耦合等离子体质谱检测进行的表征
Bio-Functional, Lanthanide-Labeled Polymer Particles by Seeded Emulsion Polymerization and their Characterization by Novel ICP-MS Detection.
作者信息
Thickett Stuart C, Abdelrahman Ahmed I, Ornatsky Olga, Bandura Dmitry, Baranov Vladimir, Winnik Mitchell A
机构信息
Chemistry Department, The University of Toronto, 80 St George Street Toronto ON CANADA M5S3H6. ; Tel: +1 416 978 6495.
出版信息
J Anal At Spectrom. 2010;25(3):269-281. doi: 10.1039/b916850h.
We present the synthesis and characterization of monodisperse, sub-micron poly(styrene) (PS) particles loaded with up to and including 10(7) lanthanide (Ln) ions per particle. These particles have been synthesized by seeded emulsion polymerization with a mixture of monomer and a pre-formed Ln complex, and analyzed on a particle-by-particle basis by a unique inductively coupled plasma mass cytometer. Seed particles were prepared by surfactant-free emulsion polymerization (SFEP) to obtain large particle sizes in aqueous media. Extensive surface acid functionality was introduced using the acid-functional initiator ACVA, either during seed latex synthesis or in the second stage of polymerization. The loading of particles with three different Ln ions (Eu, Tb, and Ho) has proven to be close to 100 % efficient on an individual and combined basis. Covalent attachment of metal-tagged peptides and proteins such as Neutravidin to the particle surface was shown to be successful and the number of bound species can be readily determined. We believe these particles can serve as precursors for multiplexed, bead-based bio-assays utilizing mass cytometric detection.
我们展示了单分散亚微米级聚（苯乙烯）（PS）颗粒的合成与表征，每个颗粒负载多达10⁷个镧系（Ln）离子，包括10⁷个。这些颗粒通过种子乳液聚合，使用单体和预先形成的Ln络合物混合物合成，并通过独特的电感耦合等离子体质谱细胞仪逐个颗粒进行分析。种子颗粒通过无表面活性剂乳液聚合（SFEP）制备，以在水性介质中获得大粒径。在种子胶乳合成期间或聚合的第二阶段，使用酸官能引发剂ACVA引入大量表面酸官能团。已证明用三种不同的Ln离子（铕、铽和钬）负载颗粒在单个和组合基础上的效率接近100%。金属标记的肽和蛋白质（如中性抗生物素蛋白）与颗粒表面的共价连接已证明是成功的，并且结合物种的数量可以很容易地确定。我们相信这些颗粒可以作为利用质谱细胞术检测的多重、基于微珠的生物测定的前体。
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