Morgan Edward, Varro Rudi, Sepulveda Homero, Ember Julia A, Apgar John, Wilson Jerry, Lowe Larry, Chen Roy, Shivraj Lalita, Agadir Anissa, Campos Roberto, Ernst David, Gaur Amitabh
BD Biosciences, San Diego, CA 92121, USA.
Clin Immunol. 2004 Mar;110(3):252-66. doi: 10.1016/j.clim.2003.11.017.
The introduction of flow cytometric bead-based technology has added a new approach for investigators to simultaneously measure multiple analytes in biological and environmental samples. This new technology allows for (1) evaluation of multiple analytes in a single sample; (2) utilization of minimal sample volumes to glean data; (3) reproducibility and results comparative with previous experiments; (4) direct comparison with existing assays; and (5) a more rapid evaluation of multiple samples in a single platform. The cytometric bead array (CBA) system enables simultaneous measurement of multiple analytes in sample volumes too small for traditional immunoassays. Results have been presented for the analysis of a variety of human cytokines. In addition, the technology allows for the design and creation of assays to measure a variety of analytes including inflammatory mediators, chemokines, immunoglobulin isotypes, intracellular signaling molecules, apoptotic mediators, adhesion molecules, and antibodies. New initiatives put forward by the Human Genome Project and the FDA require the development and use of assays for the rapid simultaneous quantitation of multiple analytes. The CBA technology provides the ability to quantify multiple proteins within a given sample, with precision and consistency.
基于流式细胞术微珠的技术的引入，为研究人员在生物和环境样品中同时测量多种分析物增添了一种新方法。这项新技术能够实现：（1）在单个样品中评估多种分析物；（2）利用极少的样品体积来收集数据；（3）具有可重复性且结果可与先前实验进行比较；（4）可与现有检测方法直接比较；（5）在单个平台上更快速地评估多个样品。细胞计数微珠阵列（CBA）系统能够在样品体积过小而无法进行传统免疫检测的情况下，同时测量多种分析物。目前已经展示了对多种人类细胞因子进行分析的结果。此外，该技术还能设计和创建用于测量多种分析物的检测方法，这些分析物包括炎症介质、趋化因子、免疫球蛋白同种型、细胞内信号分子、凋亡介质、黏附分子和抗体。人类基因组计划和美国食品药品监督管理局提出的新倡议要求开发和使用能够快速同时定量多种分析物的检测方法。CBA技术能够精确且一致地在给定样品中对多种蛋白质进行定量。
