College of Chemical Engineering, Nanjing Forestry University, Nanjing, China.
Nat Prod Res. 2010 Apr;24(6):590-8. doi: 10.1080/14786410903194472.
The biological activities and characterisation of proanthocyanidins (PAs) from the barks of Pinus massonian and Acacia mearnsii have been studied in our research. The free-radical scavenging activity of the PAs was measured by means of the DPPH method, and it was clear that the PA product had a strong radical scavenging ability. Furthermore, anti-tumour activities of PAs on different human cancer cells were investigated. The results indicated that PAs from the bark of A. mearnsii had better anti-tumour activities than those from the bark of P. massonian, and the PAs extracted from the ethyl acetate fraction had better anti-tumour activities than those from the water fraction. PAs from the bark of A. mearnsii in an ethyl acetate fraction (PAE) had an effective inhibition on human mammary cancer cells (MDA- MB-231) and human liver cancer cells (BEL-7402), a weak effect on human cervical cancer cells (Hela), but no effect on human lung cancer cells (A549); while the PAs from the bark of A. mearnsii in a water fraction (PAW) had weak effect on MDA- MB-231, Hela and A549, but no effect on BEL-7402. PAs from the bark of P. massonian in ethyl acetate fraction (PPE) had weak effect on Hela and BEL-7402; whereas the PAs from the bark of P. massonian in a water fraction (PPW) had a weak effect on Hela, but no effect on the other cells. PAs were characterised by HPLC- ESI-MS analysis and the PA dimers and trimers were identified, respectively.
我们研究了马尾松和金合欢树皮中原花青素（PAs）的生物活性和特性。通过 DPPH 法测定了 PAs 的自由基清除活性，结果表明 PA 产物具有很强的自由基清除能力。此外，还研究了 PAs 对不同人癌细胞的抗肿瘤活性。结果表明，金合欢树皮中的 PAs 比马尾松树皮中的 PAs 具有更好的抗肿瘤活性，乙酸乙酯部分提取的 PAs 比水部分提取的 PAs 具有更好的抗肿瘤活性。金合欢树皮中的 PAs 在乙酸乙酯部分（PAE）对人乳腺癌细胞（MDA-MB-231）和人肝癌细胞（BEL-7402）有有效抑制作用，对人宫颈癌细胞（Hela）作用较弱，但对人肺癌细胞（A549）无作用；而金合欢树皮中的 PAs 在水部分（PAW）对 MDA-MB-231、Hela 和 A549 作用较弱，但对 BEL-7402 无作用。马尾松树皮中的 PAs 在乙酸乙酯部分（PPE）对 Hela 和 BEL-7402 作用较弱；而马尾松树皮中的 PAs 在水部分（PPW）对 Hela 作用较弱，但对其他细胞无作用。采用 HPLC-ESI-MS 分析对 PAs 进行了表征，分别鉴定了 PA 二聚体和三聚体。
