SLCL研讨会抗体的血型反应性
Blood group reactivities of SLCL-workshop antibodies.
作者信息
Ernst M, Sonneborn H H
机构信息
BIOTEST AG, Research Department, Offenbach, Germany.
出版信息
Br J Cancer Suppl. 1991 Jun;14:37-8.
We have tested all 98 workshop antibodies for blood group antigen reactivity. In initial experiments we used a routine agglutination technique with A1LEb and BLea erythrocytes to screen for reactivity with most of the possible antigens. Except for WS78 which reacted with both types of erythrocyte and WS92 which reacted with A1Leb, there was only very weak or no reactivity. We therefore used indirect immunofluorescence and a cell analyser to increase sensitivity and tested a variety of erythrocytes expressing different blood group antigens. WS78 was strongly reactive against all types of erythrocyte tested (96-99% of the cells). WS92 appeared to react with blood group A1. WS27 detected an antigen present on all erythrocytes but expressed weakly (19-21% of the cells).
我们已对所有98种车间抗体进行了血型抗原反应性测试。在最初的实验中，我们使用常规凝集技术，以A1LEb和BLea红细胞来筛查与大多数可能抗原的反应性。除了与两种类型红细胞均有反应的WS78以及与A1Leb有反应的WS92外，其余反应都非常微弱或无反应。因此，我们采用间接免疫荧光法和细胞分析仪来提高灵敏度，并测试了多种表达不同血型抗原的红细胞。WS78对所有测试的红细胞类型均有强烈反应（96 - 99%的细胞）。WS92似乎与A1血型有反应。WS27检测到所有红细胞上均存在的一种抗原，但表达较弱（19 - 21%的细胞）。
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