Key Laboratory of Bioactive Materials, Ministry of Education, Department of Microbiology, Nankai University, Tianjin 300071, China.
J Environ Sci (China). 2010;22(1):150-4. doi: 10.1016/s1001-0742(09)60086-3.
An 8-month survey was conducted to detect and quantify enteroviruses in Tianjin coastal seawaters of Bohai Bay to assess coastal water quality. Ten water samples were collected from Bohai Bay for the detection and quantification of enteroviruses by conventional reverse transcription polymerase chain reaction (RT-PCR) and SYBR Green real-time quantitative RT-PCR (qRT-PCR). Total viral nucleic acid was extracted from 500 mL of seawater samples concentrated by Centricon plus-70 centrifugal filter devices. The viral recovery rate was 29.1% based on viral seeding study. The centrifugal ultrafiltration method applied is effective for viral recovery from small volume of polluted water, which may have broader applications to monitoring human virus in aquatic environment. Our results indicated that there was a severe viral contamination in seawater of Bohai Bay. Enteroviruses were detected at concentrations ranging from 1.7 x 10(6) to 6.3 x 10(7) copies/L by qRT-PCR. Sequencing analyses identified that all of the twenty clones as poliovirus type 2. This is the first quantitative report of human viruses in coastal waters of a metropolitan city in China. This study emphasized the importance for the local and central governments to monitor and assess the water quality.
对渤海湾天津近岸海域海水中肠道病毒进行了为期 8 个月的检测和定量分析，以评估近岸海域水质。采集了 10 个海水样本，采用常规逆转录聚合酶链反应（RT-PCR）和 SYBR Green 实时定量 RT-PCR（qRT-PCR）检测和定量肠道病毒。用 Centricon plus-70 离心超滤器浓缩 500 mL 海水样品，提取总病毒核酸。病毒接种研究表明，病毒回收率为 29.1%。应用的离心超滤法对于从小体积污染水中回收病毒是有效的，这可能对监测水生环境中的人类病毒有更广泛的应用。我们的研究结果表明，渤海湾近岸海域海水中存在严重的病毒污染。qRT-PCR 检测到肠道病毒的浓度范围为 1.7 x 10(6) 至 6.3 x 10(7) 拷贝/L。测序分析表明，20 个克隆均为脊髓灰质炎病毒 2 型。这是中国特大城市近岸海域人类病毒的首次定量报告。本研究强调了地方和中央政府监测和评估水质的重要性。
