University of Manitoba, Department of Biochemistry and Medical Genetics, Winnipeg, Manitoba, Canada.
FEBS Lett. 2010 Jun 3;584(11):2218-24. doi: 10.1016/j.febslet.2010.04.022. Epub 2010 Apr 14.
Products of the steroid receptor RNA activator (SRA1) gene have the unusual property to function both at the RNA and the protein levels. SRA-RNA has long been known to increase the activity of multiple nuclear receptors. It has more recently been proposed than steroid receptor RNA activator protein (SRAP) also modulates steroid receptors activity. Herein, we show for the first time that SRAP physically interacts with multiple transcription factors and is recruited to specific promoter regions. Artificially recruiting SRAP to the promoter of a luciferase reporter gene under the control of the strong transcriptional activator VP16 leads to a decrease in transcription. Altogether we propose that SRAP could be a new transcriptional regulator, able to function as a repressor through direct association with promoters.
类固醇受体 RNA 激活物（SRA1）基因的产物具有在 RNA 和蛋白质水平上均发挥作用的不寻常特性。长期以来，SRA-RNA 一直被认为可以提高多种核受体的活性。最近有人提出，类固醇受体 RNA 激活蛋白（SRAP）也可以调节类固醇受体的活性。在此，我们首次表明，SRAP 与多个转录因子发生物理相互作用，并被募集到特定的启动子区域。人为地将 SRAP 募集到受强转录激活剂 VP16 控制的荧光素酶报告基因的启动子上，会导致转录减少。总之，我们提出 SRAP 可能是一种新的转录调节剂，能够通过与启动子直接结合而发挥抑制作用。
