Tanaka T, Hirota Y, Sohmiya K, Nishimura S, Kawamura K
Department of Internal Medicine, Osaka Medical College, Takatsuki, Japan.
Clin Biochem. 1991 Apr;24(2):195-201. doi: 10.1016/0009-9120(91)90571-u.
A competitive enzyme immunoassay (C-EIA) was developed for the measurement of serum and urinary levels of human heart fatty acid-binding protein (hh-FABP), and the appearance and time-course changes of hh-FABP levels were evaluated in patients with acute myocardial infarction (AMI). Control serum and urinary hh-FABP levels, which were determined in 86 serum and 42 urine samples from 86 patients without AMI, were found to range between 0 and 2.8 ng/mL. Serial determinations performed on 11 patients with AMI demonstrated that hh-FABP levels were significantly elevated in the first serum and urine samples obtained within 14 h of the onset of clinical symptoms. Two serum and 2 urine samples obtained only 1.5 h after the onset of symptoms already showed elevated hh-FABP levels, while in the same serum samples the activity of the myocardial-specific isoenzyme of creatine kinase (CK-MB) was still normal. Maximal serum and urinary hh-FABP levels appeared between 5 and 10 h after symptoms developed, and fell sharply towards normal thereafter. The hh-FABP levels in serum and urine both peaked earlier than the elevation of CK-MB activity in serum. The presence of hh-FABP in serum and/or urine seems to be a marker for myocardial damage and could be used as a useful tool for the early diagnosis of AMI.
开发了一种竞争性酶免疫测定法（C-EIA）用于检测人心脏脂肪酸结合蛋白（hh-FABP）的血清和尿液水平，并评估急性心肌梗死（AMI）患者中hh-FABP水平的出现及时间进程变化。在86例无AMI患者的86份血清和42份尿液样本中测定的对照血清和尿液hh-FABP水平在0至2.8 ng/mL之间。对11例AMI患者进行的系列测定表明，在临床症状发作后14小时内采集的首份血清和尿液样本中，hh-FABP水平显著升高。症状发作仅1.5小时后采集的2份血清和2份尿液样本中，hh-FABP水平已升高，而在相同血清样本中，肌酸激酶心肌特异性同工酶（CK-MB）的活性仍正常。血清和尿液中hh-FABP的最高水平出现在症状出现后5至10小时，此后急剧降至正常。血清和尿液中的hh-FABP水平均比血清中CK-MB活性升高的时间更早达到峰值。血清和/或尿液中hh-FABP的存在似乎是心肌损伤的标志物，可作为AMI早期诊断的有用工具。
