Fukushima Kenji, Momose Mitsuru, Kanaya Kazuko, Kaimoto Yoko, Higuchi Takahiro, Yamamoto Atsushi, Nakao Risako, Matsuo Yuka, Nagao Michinobu, Kuji Ichiei, Abe Koichiro
Department of Nuclear Medicine, Saitama Medical University International Medical Center, Japan.
Department of Diagnostic Imaging and Nuclear Medicine, Tokyo Women's Medical University, Japan.
Ann Nucl Cardiol. 2022;8(1):14-20. doi: 10.17996/anc.21-00146. Epub 2022 Aug 31.
: Heart-type fatty acid binding protein (H-FABP) is primary transporter of free fatty acid and plays an important role in myocardial metabolism, which is characterized by high specificity and rapid appearance under ischemic condition. The objective of this study was to clarify the usefulness of imaging study of targeting H-FABP appearance using radio-labeled antibody, and correlation with myocardial fatty acid metabolism and perfusion in acute reperfusion ischemia. : Wistar rats were allotted to sham-operated control group (sham; n=4), ischemia non-reperfused group (IG; n=5), and ischemia-reperfusion group (RG; n=5). Ligation of left coronary artery (LCA) was performed for IG and RG. 20 min of ischemia was followed by 60min of reperfusion for RG. I labeled anti H-FABP antibody (anti H-FABP), BMIPP and Tc-sestamibi (MIBI) was injected intravenously. Multi-tracer digital autoradiogram was performed using µ-imager. The ratio of radioactivity in LCA related (culprit) area to the inferior (remote) area (target uptake ratio=TUR) was generated. : In sham group, no visually detectable accumulation was observed for the anti H-FABP image, and TUR and TUR were equivalent to 1. In IG, TUR and TUR were remarkably low (0.12±0.01, 0.24±0.07). In RG, TUR was significantly lower (0.20±0.03, p<0.05 vs. other groups). However, TUR was significantly higher (2.78±1.28, p<0.05) compared to the sham and IG, whereas anti H-FABP showed markedly higher ratio in the reperfused area compared to the sham and IG (3.43±0.73 vs. 0.31±0.13 and 1.09±0.07 for IG and sham; p<0.05, and <0.01, respectively). : Anti H-FABP accumulated specifically in reperfused area under acute ischemia, and it accorded to the area where fatty acid metabolism was activated. This study has shown the future potential for clinical application imaging of acute coronary syndrome.
心脏型脂肪酸结合蛋白（H-FABP）是游离脂肪酸的主要转运蛋白，在心肌代谢中起重要作用，其特点是特异性高且在缺血状态下快速出现。本研究的目的是阐明使用放射性标记抗体靶向H-FABP显像的实用性，以及与急性再灌注缺血时心肌脂肪酸代谢和灌注的相关性。：将Wistar大鼠分为假手术对照组（假手术组；n = 4）、缺血未再灌注组（IG组；n = 5）和缺血再灌注组（RG组；n = 5）。对IG组和RG组进行左冠状动脉（LCA）结扎。RG组在缺血20分钟后再灌注60分钟。静脉注射I标记的抗H-FABP抗体（抗H-FABP）、BMIPP和锝- sestamibi（MIBI）。使用μ-成像仪进行多示踪数字放射自显影。计算LCA相关（罪犯）区域与下壁（远隔）区域的放射性比值（靶摄取率=TUR）。：在假手术组，抗H-FABP图像未观察到视觉上可检测到的积聚，TUR和TUR等于1。在IG组，TUR和TUR显著降低（0.12±0.01，0.24±0.07）。在RG组，TUR显著降低（0.20±0.03，与其他组相比p<0.05）。然而，与假手术组和IG组相比，TUR显著升高（2.78±1.28，p<0.05），而抗H-FABP在再灌注区域的比值与假手术组和IG组相比显著更高（IG组和假手术组分别为3.43±0.73对0.31±0.13和1.09±0.07；p<0.05和<0.01）。：抗H-FABP在急性缺血时特异性积聚在再灌注区域，且与脂肪酸代谢激活区域一致。本研究显示了急性冠状动脉综合征临床应用成像的未来潜力。
