从澳大利亚一头健康公牛的精液中分离出5型牛疱疹病毒。
Isolation of bovine herpesvirus type 5 from the semen of a healthy bull in Australia.
作者信息
Diallo I S, Hewitson G R, Hoad J, Turner S, Corney B G, Rodwell B J
机构信息
Biosecurity Sciences Laboratory, Department of Primary Industries and Fisheries, Locked Mail Bag 4, Moorooka, Queensland 4105, Australia.
出版信息
Aust Vet J. 2010 Mar;88(3):93-5. doi: 10.1111/j.1751-0813.2009.00545.x.
Artificial insemination is widely used in the cattle industry and a major challenge is to ensure that semen is free of infectious agents. A healthy donor bull was tested for freedom from infectious agents. A bovine herpesvirus was isolated in testis cells and identified as bovine herpesvirus type 5 (BoHV-5) by polymerase chain reaction and by direct amplicon sequencing. The amplicon sequence shared 100% similarity with the published sequence of BoHV-5. This is the first report in Australia of BoHV-5 in semen. The implications of this finding are discussed.
人工授精在养牛业中广泛应用，而一项主要挑战是确保精液无感染因子。对一头健康的供体公牛进行了无感染因子检测。在睾丸细胞中分离出一种牛疱疹病毒，并通过聚合酶链反应和直接扩增子测序鉴定为牛疱疹病毒5型（BoHV-5）。扩增子序列与已发表的BoHV-5序列具有100%的相似性。这是澳大利亚首次关于精液中BoHV-5的报道。本文讨论了这一发现的意义。
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