Jiangsu Provincial Key Lab of Pulp and Paper Science and Technology, Nanjing Forestry University, Nanjing 210037, China; Department of Forest Biomaterials, North Carolina State University, Raleigh, NC 27695, USA.
Jiangsu Provincial Key Lab of Pulp and Paper Science and Technology, Nanjing Forestry University, Nanjing 210037, China.
Bioresour Technol. 2014 Jun;161:63-8. doi: 10.1016/j.biortech.2014.03.024. Epub 2014 Mar 13.
Pretreatment of wheat straw with a combination of sodium carbonate (Na2CO3) or sodium hydroxide (NaOH) with oxygen (O2) 0.5MPa was evaluated for its delignification ability at relatively low temperature 110°C and for its effect on enzymatic hydrolysis efficiency. In the pretreatment, the increase of alkali charge (as Na2O) up to 12% for Na2CO3 and 6% for NaOH, respectively, resulted in enhancement of lignin removal, but did not significantly degrade cellulose and hemicellulose. When the pretreated solid was hydrolyzed with a mixture of cellulases and hemicellulases, the sugar yield increased rapidly with the lignin removal during the pretreatment. A total sugar yield based on dry matter of raw material, 63.8% for Na2CO3-O2 and 71.9% for NaOH-O2 was achieved under a cellulase loading of 20FPU/g-cellulose. The delignification efficiency and total sugar yield from enzymatic hydrolysis were comparable to the previously reported results at much higher temperature without oxygen.
预处理小麦秸秆与碳酸钠（Na2CO3）或氢氧化钠（NaOH）与氧气（O2）0.5MPa 的组合，在相对较低的温度 110°C 下评估其脱木质素能力，并评估其对酶水解效率的影响。在预处理中，碱负荷（以 Na2O 计）分别增加到 12%的 Na2CO3 和 6%的 NaOH，导致木质素去除增加，但不会显著降解纤维素和半纤维素。当用纤维素酶和半纤维素酶混合物水解预处理的固体时，随着预处理过程中木质素的去除，糖产率迅速增加。在纤维素酶用量为 20FPU/g-纤维素的情况下，基于原料干物质的总糖产率，Na2CO3-O2 为 63.8%，NaOH-O2 为 71.9%。在没有氧气的情况下，在更高的温度下，脱木质素效率和酶水解的总糖产率与之前报道的结果相当。
