Shanghai Ocean University, PR China.
Comp Biochem Physiol Part D Genomics Proteomics. 2010 Jun;5(2):116-23. doi: 10.1016/j.cbd.2010.02.003. Epub 2010 Feb 20.
Grass carp is the third largest aquaculture species in global production. However, genomic research of this species has been limited. To identify immune-related genes in grass carp, a normalized full-length cDNA library was constructed from head kidney tissues, and 6432 randomly selected clones were sequenced. 5289 high quality expressed sequence tags (EST) were generated and assembled into 2687 unigenes. Among them, 1585 unigenes showed significant similarity with known sequences in public databases, whereas the remaining 1102 unigenes appeared to be novel sequences with unknown functions. In particular, 136 immune-related genes were identified to encode immunoglobulins, FcRgamma, IFN-related proteins, various CD markers, MHCs, complements and other important immune-related factors; a majority of these genes are reported in grass carp for the first time. Sequence analysis indicated that grass carp has at least three subtypes of immunoglobulin light chains, namely L1, L2 and L3. Furthermore, FCRgamma was found to broadly express in different tissues. Our study constitutes the first EST analysis of lymphatic tissue in grass carp, and could pave the way for further research of immune-related genes and functional genomics in grass carp.
草鱼是全球产量第三大的水产养殖品种。然而，该物种的基因组研究一直受到限制。为了鉴定草鱼中的免疫相关基因，我们从头肾组织构建了一个标准化的全长 cDNA 文库，并对 6432 个随机挑选的克隆进行了测序。共产生了 5289 条高质量的表达序列标签 (EST)，并将其组装成 2687 条非冗余基因。其中，1585 个基因与公共数据库中的已知序列具有显著相似性，而其余 1102 个基因似乎是具有未知功能的新序列。特别地，我们鉴定出了 136 个免疫相关基因，它们编码免疫球蛋白、FcRγ、IFN 相关蛋白、各种 CD 标记物、MHCs、补体和其他重要的免疫相关因子；其中大多数基因在草鱼中是首次报道。序列分析表明，草鱼至少有三种免疫球蛋白轻链亚型，即 L1、L2 和 L3。此外，我们发现 FCRγ在不同组织中广泛表达。我们的研究首次对草鱼淋巴组织进行了 EST 分析，为草鱼免疫相关基因和功能基因组学的进一步研究奠定了基础。
